Slide shows combined and modified from: http://gbs.glenbrook.k12.il.us/Academics/gbssci/bio/apbio/Lecture/lecture.htm; h

1. AP BIOLOGY Chapter 6Cell Structure & FunctionWHAT’S NEW you didn’t learn in BIO I? Slide shows combined and modified from:http://gbs.glenbrook.k12.il.us/Academics/gbssci/bio/apbio/Lecture/lecture.htm; http://www.explorebiology.com/ http://home.att.net/~tljackson/neville.html

3. http://cellbio.utmb.edu/cellbio/nuclear_envelope.htm NUCLEAR ENVELOPE DOUBLE MEMBRANE isfused in spots formingNUCLEAR PORES NUCLEAR LAMINA- netlike array of protein filaments on nuclear side of envelope that maintains the shape of the nucleus (Play a role in reforming nuclear membrane after cell division,if you inject antibodies to lamina proteins, nucleus can’t reform after mitosis)

4. Nucleus Nucleus 1 µm Nucleolus Chromatin Nuclear envelope: Inner membrane Outer membrane Nuclear pore Pore complex Rough ER Surface of nuclear envelope. 1 µm Ribosome 0.25 µm Close-up of nuclear envelope Nuclear lamina (TEM). Pore complexes (TEM). NUCLEAR ENVELOPE Figure 6.10

8. ENDOMEMBRANE SYSTEM Regulates protein traffic and performs metabolic functions in the cell Includes:Plasma membraneNuclear membraneEndoplasmic reticulumGolgi apparatusVacuolesLysosomes

10. INSULIN being released by pancreas cells using exocytosis http://fig.cox.miami.edu/~cmallery/255/255ion/fig14x26.jpg

12. Golgi apparatusCisternae = Flattened membrane sacs (look like stacked pancakes)2 sides = 2 functions • cis = (receives vesicles by fusion) • trans = buds off vesicles to send to other places (shipping face)

14. Animation from: http://www.franklincollege.edu/bioweb/A&Pfiles/week04.html See a Golgi movie

15. EVERYTHING’S CONNNECTED!

16. LYSOSOMES (common in animal cells but rare in plant cells) Contain hydrolytic enzymes for intracellular digestion • Food (Phagocytosis) • Damaged organellesAUTOPHAGY ~ “eating self” See movie

18. LYSOSOMES: Uncontrolled release of lysosome contents into the cytoplasm can also cause cell death (necrosis) • APOPTOSIS (self-destruct mechanism)“cell suicide” Embryonic development Removes damaged cells Immune response Cancer cells and AIDS virus override self-destruct signals

19. WHITE BLOOD CELLS USE LYSOSOMES TODIGEST ENGULFED BACTERIA(Phagocytosis) http://fig.cox.miami.edu/~cmallery/255/255ion/fig14x28.jpg

21. ANIMAL VACUOLES & VESICLES“transport vehicles” • FOOD VACUOLES Phagocytosis/ fuse with lysosomes • CONTRACTILE VACUOLES Freshwater organisms pump out excess water http://www.microscopy-uk.org.uk/mag/imgjun99/vidjun1.gif

22. PLANT VACUOLES (Central Vacuole) • Surrounded by membrane = TONOPLASTSelectively permeable – controls what goes in & out • STORAGE • Water • Stockpile proteins/inorganic ions • Deposit metabolic byproducts • Store pigments • Store defensive compounds against herbivores

23. Other cell parts with membranes that are NOT part of the Endomembrane system: • Mitochondria • Plastids • Peroxisomes

26. MITOCHONDRIA- DOUBLE MEMBRANE Outer membrane Inner membrane (CRISTAE) increases surface area for chemical reactions Intermembrane space=Space between inner membrane & outer membrane MATRIX = Space inside cristae foldscontains DNA, enzymes, ribosomes

29. Chloroplasts – site for photosynthesis in plants • DOUBLE MEMBRANE • Has own DNA • Thylakoids – membrane sacs containing light-capturing pigments • Grana – closed compartments of stacked thylakoid membranes • Stroma – fluid surrounding thylakoids

30. PLASTIDS CHLOROPLASTS- contain pigment chlorophyll for photosynthesis CHROMOPLASTS- contain pigments that give fruits and flowers colors AMYLOPLASTS- store starch (amylose) in roots and tubers (colorless) http://www.jonathanwald.com/800x600/images/Red-Apple.jpghttp://en.wikipedia.org/wiki/Image:Potato_-_Amyloplasts.jpg

31. Who else has a circular chromosome not found within a nucleus?

32. http://en.wikipedia.org/wiki/Lynn_Margulis Endosymbiotic Theory • Originally proposed in early 1900’s • Idea reintroduced in 1963 by Lynn Margulis • Suggests that engulfed prokaryotes sharedsymbiotic relationship with host cell • Advantages for both: ~ one supplies energy ~ other raw materials & protection

33. ENDOSYMBIOTIC THEORY See a movie about ENDOSYMBIOTIC THEORY http://www.mun.ca/biology/scarr/Endosymbiosis_theory.gif

34. Evidence for Endosymbiotic theory 1. Mitochondria and chloroplasts have circular DNA like bacteria. 2. Mitochondria and chloroplasts have ribosomes whose size and structure resemble bacterial ribosomes. 3. Mitochondria and chloroplasts replicate independently of cell division using binary fission like bacteria. 4. Inner membranes of mitochondria and chloroplasts have a composition similar to bacterial membranes.

35. PEROXISOMES Other digestive enzyme sacsin both plants and animals NOT part of endomembrane system(proteins come from cytosol) In fat storing seeds (called GLYOXYSOMES)Break down fatty acids → sugars transport to mitochondria for energy In LIVER CELLSHelp detoxify alcohol & other poisons PRODUCE HYDROGEN PEROXIDE (also a TOXIN)but have enzyme (CATALASE) to break this down H2O2 → H2O + O2