80 likes | 166 Views
e. b. a. 1. 2. 1 2. 1 2. 400 bp. OPN (400 bp). OPN. 300 bp. OPN/ DAPI. 200 bp. GAPDH (188 bp). Actin. MEF. WT KO. Hyp - + Time (hr) 0 16. MCF-7. Hyp - +
E N D
e b a 1 2 1 2 12 400 bp OPN (400 bp) OPN 300 bp OPN/ DAPI 200 bp GAPDH (188 bp) Actin MEF WT KO Hyp - + Time (hr) 0 16 MCF-7 Hyp - + Time (hr) 0 16 MCF-7 d c 1 2 3 4 5 1 2 3 HIF1α OPN HIF1β f 1 2 3 Actin Actin HIF1α C Coni OPNi Nor + + - - - Hyp - - + + + Coni - + + - - OPNi - - - + - β3i - - - - + MDA-MB-231 MCF-7 1 2 3 Actin β3-Integrin MCF-7 Hyp + + + rhOPN - 0.25 0.5 (μM) Actin C Coni β3i MDA-MB-231 Supp.Fig. S1 Raja et al.
Hyp Nor 1 2 Hyp Nor 1 2 3 1 2 3 OPN p-Akt HIF1α Actin Akt Actin 231 OPNshRNA p-p65 MDA-MB-231 VEGF p65 Actin 231 231 OPNshRNA 231 231 OPNshRNA MDA-MB-231 MDA-MB-231 a b c Supp.Fig. S2 Raja et al.
a Nor Hyp Hyp+ Coni Hyp + OPNi T=0 h T=16 h Hyp + β3 i Hyp +IgG Hyp +OPN Neu Ab c T=0 h % Wound Closure T=16 h MDA-MB-231 MDA-MB-231 b T=0 h T=16 h Hypoxia + + + rhOPN (0.25 µM) - + - OPN shRNA - - + * * Hyp + + + rh OPN - + - (0.25 µM) OPN shRNA - - + Supp.Fig.S3 Raja et al.
Nor T=0h T=16h Hyp T=0h T=16h OPN (0.5μM) - + + + - Coni - - + - - HIF1α i - - - + + MDA-MB-231 Supp.Fig.S4 Raja et al.
a Nor Hyp Coni Coni OPNiβ3i % Migration MDA-MB-231 Nor + - - - Hyp - + + + Coni + + - - OPNi - - + - β3 i - - - + c b Nor Hyp Hyp+ Coni * ** MDA-MB-231 Hyp +OPNi Hyp+ β3i Migration Invasion Supp.Fig.S5 Raja et al.
MCF-7 MCF-OPN-a HIF2α DAPI Hyp + + (16 h) Supp.Fig.S6 Raja et al.
Correlation coefficient of OPN and HIF1α expression in human breast cancer Supplementary Table S1 Raja et al.
Correlation coefficient of OPN, HIF1α and HIF2α expression in human breast cancer Supplementary Table S2 Raja et al.