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PREPARATION OF A BACTERIAL SMEAR SPECIAL STAINING TECHNIQUES

PREPARATION OF A BACTERIAL SMEAR SPECIAL STAINING TECHNIQUES. ASEPTIC PROCECURE FOR MICROBE REMOVAL. http://www.youtube.com/watch?v=0odxJy0nR9s&feature=related. ASEPTIC PROCECURE FOR MICROBE REMOVAL FOR PREPARATION OF A BACTERIAL SMEAR.

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PREPARATION OF A BACTERIAL SMEAR SPECIAL STAINING TECHNIQUES

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  1. PREPARATION OF A BACTERIAL SMEARSPECIAL STAINING TECHNIQUES

  2. ASEPTIC PROCECURE FOR MICROBE REMOVAL http://www.youtube.com/watch?v=0odxJy0nR9s&feature=related

  3. ASEPTIC PROCECURE FOR MICROBE REMOVAL FOR PREPARATION OF A BACTERIAL SMEAR

  4. http://www.youtube.com/watch?v=HxjN2mBLGCE&feature=related http://www.youtube.com/watch?v=n-HHI4E1LhI&feature=related

  5. SPECIMEN PREPARATION FOR LIGHT MICROSCOPY • PREPARATION OF A BACTERIAL SMEAR

  6. STAINING • SPECIAL STAINING TECHNIQUES • Negative Staining for Capsules • Capsules • Determinants for virulence in some genera of bacteria • Capsule Staining • Preparation of a bacterial smear along with negative staining w/ Nigrosin or India Ink • Counterstain w/ Crystal Violet

  7. STAINING • SPECIAL STAINING TECHNIQUES • Negative Staining for Capsules • Preparing a bacterial smear for Capsule Staining

  8. STAINING • SPECIAL STAINING TECHNIQUES • Negative Staining for Capsules • Capsule Stain Results

  9. STAINING • SPECIAL STAINING TECHNIQUES • Endospore Stain • Genera of bacteria that produce endospores • Bacillus & Clostridium • Other genera: Desulfotomaculum, Sporosarcina, Sporolactobacillus, Oscillospira,Thermoactinomyces • Schaeffer-Fulton Method for Endospore Staining • Preparation of a Bacterial Smear-Air dry • Application of Primary Dye (Malachite Green) w/ concurrent application of heat • Allow slide to cool • Rinse w/ water • Application of Counterstain (Safranin)

  10. http://www.tgw1916.net/video_pages/Spore.html http://www.answers.com/topic/endospore

  11. STAINING http://www.tgw1916.net/movies.html • SPECIAL STAINING TECHNIQUES • Endospore Stain Results • Flagellar Stain • Uses mordant and carbolfuchsin stain to increase flagellar diameter until resolved by light microscope http://www.answers.com/topic/endospore

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