1 / 7

Material compatibility

Material compatibility. What are these tubes tubes made of?. Polypropylene High chemical and thermal resistance (autoclavable) Polystyrene Cheaper clear plastic Usually for disposable applications Polycarbonate High strength – high speed spins Less chemical resistance.

oren
Download Presentation

Material compatibility

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Material compatibility

  2. What are these tubes tubes made of? • Polypropylene • High chemical and thermal resistance (autoclavable) • Polystyrene • Cheaper clear plastic • Usually for disposable applications • Polycarbonate • High strength – high speed spins • Less chemical resistance

  3. Sample preparation tubes • Color tubes • Can leech dyes • Don’t use for MS or optical measurements • Sterile • Irradiated (gamma radiation) • RNAse/DNAse free • Low retention (Lo Bind) tubes • Coated to bind less protein • Recommended for dilute protein solutions • Proteomic samples • Glass vials • Amber glass (light sensitive samples) • Silanized glass (coating)

  4. Gas cylinders & regulators • Brass • 70% copper, 30% zinc • Stainless steel • 304 - 18% chromium, 8% nickel • 316 - 16% chromium, 8% nickel, 2% molybdenum • More corrosion resistance & more $$$ • Gas purity • 2.0 – 99% • 4.0 – 99.99% • 5.5 – 99.9995%

  5. Water • Industrial water • Untreated (not potable) • Deionized (DI) • Reverse osmosis • One shared tank for the wing • MilliQ • Combination of UV irradiation, ion exchange, and graphite stages • Final step through 0.22 μm membrane • May still contain a lot of plastic polymers • Optima (LC-MS) • Removal of most polymers that show up on the mass spec • Optima UPLC grade (even lower levels of background)

  6. Filters • Pore size • 0.45µm • 0.22µm (very sterile applications) or MALS • Molecular weight cut-off (MWCO) • Chemistry • Polyethersulfone (PES) • Hydrophilic – low protein binding • Regenerated cellulose • Low binding, good chemical resistance • Nylon • Some chemical resistance, binds protein • Polytetrafluoroethylene (PTFE) • Good chemical resistance • Lookup Compatibility: • https://biomedical-innovations.com/Products/Syringe-Filters/Membrane-Compatibility.aspx

  7. Useful Databases • Cole Parmer Material compability: • https://www.coleparmer.com/chemical-resistance • Plastics International database: • https://www.plasticsintl.com/chemical-resistance-chart • US plastics database: • https://www.usplastic.com/catalog/files/charts/LG%20CC.pdf • Thermo Fisher’s database: • http://tools.thermofisher.com/content/sfs/brochures/D20480.pdf

More Related