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cdkc;2-1/CDKC;1 RNAi. cdkc;2-1. 1. 3. 4. 7. 10. 14. 2. 5. 8. 9. 11. 12. 13. 6. CDKC;1. UBQ10. cyct1;5-1/CYCT1;4 RNAi. cyct1;5-1. 1. 3. 4. 7. 10. 14. 2. 5. 8. 9. 12. 6. 13. 11. CYCT1;4. UBQ10.
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cdkc;2-1/CDKC;1 RNAi cdkc;2-1 1 3 4 7 10 14 2 5 8 9 11 12 13 6 CDKC;1 UBQ10 cyct1;5-1/CYCT1;4 RNAi cyct1;5-1 1 3 4 7 10 14 2 5 8 9 12 6 13 11 CYCT1;4 UBQ10 Supplemental Figure 4. RT-PCR analysis of CDKC;1 and CYCT1;4 transcripts from RNAi plants. Semiquantitative RT-PCR analysis was performed to determine the transcript levels of CDKC;1 in the cdkc;2-1 mutant and the F1 mutant transfortants harboring a CDKC;1 RNAi construct (cdkc;2-1/CDKC;1 RNAi). The same RT-PCR analysis was performed to determine the transcript levels of CYCT1;4 in the cyct1;5-1 mutant and the F1 mutant transformants harboring a CYCT1;4 RNAi construct (cyct1;5-1/CYCT1;4 RNAi). The ArabidopsisUBQ10 gene was used as a positive internal control.