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  1. Supplementary Figure 1. A FSC threshold appropriate for murine lymphocytes and myeloid cells was set prior to collection of samples on an LSR-II flow cytometer. After collection, using FlowJo, events were gated to remove aggregates via FSC-A vs. FSC-H (left panel), then gated on cells that were CD45+ as well as negative for a viability dye only taken up only by dead cells (middle panel). Finally, cells were interrogated for expression of cell surface markers of interest using appropriate combinations of negative and positive expression patterns. For example, for evaluation of neutrophils, cells negative for T cell (TCRb), B cell (CD19 and B220), and myeloid marker (B220) were selected for further analyses (right panel) by expression of CD11b and Gr1.

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