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Plant Propagation by Cuttings. a. Kinds of cuttings 1) Stem cuttings 2) Leaf-bud cutting (single-eye) 3) Root cuttings 4) Leaf cuttings b. Totipotency -The ability of an individual cell to reconstitute the entire plant part and functions
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Plant Propagation by Cuttings a. Kinds of cuttings 1) Stem cuttings 2) Leaf-bud cutting (single-eye) 3) Root cuttings 4) Leaf cuttings b. Totipotency-The ability of an individual cell to reconstitute the entire plant part and functions c. Dedifferentiation-The capability of previously developed, differentiated cell to return to a meristem
Plant Propagation by Cuttings 1. Stem cuttings 2. Leaf-bud cutting (single-eye) 3. Root cuttings 4. Leaf cuttings
1. Stem Cuttings • Softwood Cuttings • Semi-Hardwood Cutting • Hardwood Cuttings • Herbaceous Cuttings
Shoftwood Cuttings - Hydrangea Butterfly cutting (top) Double-eye single node cutting (middle) Single-eye single node cuttings (bottom)
Softwood Cuttings-Peach - Actively growing shoots are used - Softwood cuttings are taken during spring and summer
Influence of IBA on Semi-Hardwood Cuttings - Cordia 1-Control, 2-50% ethanol, 3-100 ppm, 4-1000 ppm, 5-2000 ppm, 6-4000 ppm, 7-6000 ppm, 8-8000 ppm, 9-10000 ppm IBA
Semi-Hardwood Cuttings - Jojoba Treating cuttings in IBA Solution (top) Sticking IBA-treated cuttings in root substrate (bottom)
Temperature Differential Helpful for Root Formation • Rooting requires carbohydrates (energy), auxin (growth hormone), and rooting cofactors (enzymes) • Temperature differential (10 oF) between the ambient air and root zone is helpful for faster rooting Ambient Temp 60 oF Root zone Temp 70 oF
Good sanitation is important Cutting on the left was infected with Alternaria and did not root Cutting on the right was healthy and rooted well
Cutting Propagation in Jojoba • Regular Stem Cuttings • Use 4-5 nodes • Semi-hardwood cuttings • Single Node Cuttings • Double-eye single node cuttings • Single-eye single node cuttings
Rooting of 3 Different Types of Cuttings in Jojoba Regular Cuttings Single Node Cuttings Double Eye (DE) Single Eye (SE) 5-node
Single Node Cuttings Double-Eye Single Node Cutting (DE) - Healthier than SE - Less disease attacks Single-Eye Single Node Cutting (SE) - Largest no. cuttings/plant - Slower development - Higher mortality
Clonal Selection of Jojoba from Native StandsPima Canyon, Tucson, AZ
Rooting jojoba cuttings in bundles with a bottom heat system
An orchard of clonally propagated citrus plants, Bakersfield, CA
Commercial Propagation of Poinsettia Paul Ecke, Sr. – Developer of modern day poinsettias