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Gel Electrophoresis and DNA Fingerprinting

Gel Electrophoresis and DNA Fingerprinting. What is Gel Electrophoresis?. Process used to separate fragments of DNA. Steps of Gel Electrophoresis. Prepare Gel (Agar) Prepare Box Inject DNA Run electricity, electric current. What is the result?. Using dye under UV lights. Using x-ray.

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Gel Electrophoresis and DNA Fingerprinting

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  1. Gel Electrophoresis and DNA Fingerprinting What is Gel Electrophoresis?

  2. Process used to separate fragments of DNA

  3. Steps of Gel Electrophoresis Prepare Gel (Agar) Prepare Box Inject DNA Run electricity, electric current

  4. What is the result? Using dye under UV lights Using x-ray Each band is a different trait

  5. How to read a gel? • Start by reviewing paternity bands (from mom + dad) Larger Smaller Sex D1 = Real Daughter D2 = Daughter of Dad S1 = Real Son S2 =Adopted Son

  6. EXAMPLE I: WHO IS THE CRIMINAL? SUSPECT #2

  7. EXAMPLE II: WHO IS THE CRIMINAL? DINO is the CRIMINAL

  8. EXAMPLE III: WHO IS THE CRIMINAL? MRS. WHITE is the CRIMINAL

  9. Example IV: WHO IS THE CRIMINAL? JOHN

  10. BENEFITS of ELECTROPHORESIS Applications in forensic and criminal sciences Applications in genetic biology Applications in biochemistry

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