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Experiment 3: Microbiology and Experimental Design

Experiment 3: Microbiology and Experimental Design Bacteria and Fungi on a Kitchen Cutting Board (SEM x2,270) Plastic cutting board surface - rod bacteria in a smooth matrix; filamentous bacteria -smooth oval cells; fungal hyphae (orange) with textured spores

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Experiment 3: Microbiology and Experimental Design

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  1. Experiment 3: Microbiology and Experimental Design Bacteria and Fungi on a Kitchen Cutting Board (SEM x2,270)Plastic cutting board surface - rod bacteria in a smooth matrix; filamentous bacteria -smooth oval cells; fungal hyphae (orange) with textured spores © David Kunkel http://www.denniskunkel.com/

  2. Objective: • to determine whether Dial liquid antibacterial soap kills more Escherichia coli bacteria than SoftSoap non-antibacterial liquid soap

  3. Safety Scanning electron micrograph of E. coli© David Kunkel • The strain of E. coli we are using is not pathogenic. • Nevertheless, use reasonable hygienic precautions • (hand washing, no food). • TAs will discuss sterile technique and equipment.

  4. Important points to remember • Do not start centrifuging until the centrifuge is full or no one else will need it in the near future. Use any centrifuge in the lab. • Come in tomorrow to count your plates. • Do not throw trash in the autoclave bags – they are for items contaminated with bacteria only.

  5. Variability and Significance Mean height of Lakers team: 2.23 meters Std. Dev. of Lakers: 0.25 meters Mean height of Wizards team: 2.28 meters Std. Dev. of Wizards: 0.22 meters Wizards mean is 0.05 meters taller, but that difference is much smaller than the variability (SD) in players.

  6. Mean height of Lakers team: 1.82 meters Std. Dev. of Lakers: 0.25 meters Mean height of Wizards team: 2.48 meters Std. Dev. of Wizards: 0.22 meters Wizards mean is 0.66 meters taller, and that difference is larger than the variability in players.

  7. Next week Don’t forget the first test (Sep. 21-22) • Format: all multiple choice • Review learning objectives • Sample questions on Web page

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