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Promoter recognition by sigma factors

. . . '. . Promoter recognition by sigma factors. Sigma factor. RNA polymerase core enzyme. Figure from Griffiths et al (1996) Introduction to Genetic Analysis, 6th ed., WH Freeman and Co. Promoter recognition by sigma factors.

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Promoter recognition by sigma factors

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  1.   '  Promoter recognition by sigma factors Sigma factor RNA polymerase core enzyme Figure from Griffiths et al (1996) Introduction to Genetic Analysis, 6th ed., WH Freeman and Co.

  2. Promoter recognition by sigma factors Figure from Griffiths et al (1996) Introduction to Genetic Analysis, 6th ed., WH Freeman and Co.

  3. Promoter recognition by sigma factors Figure from Griffiths et al (1996) Introduction to Genetic Analysis, 6th ed., WH Freeman and Co.

  4. A Promoter recognition by sigma factors uvrB Repair DNA damage TTGTTGGCATAATTAAGTACGACGAGTAAAATTAC ATACCT recA DNArecombination CACTTGATACTGTA.TGAGCATACAGTATAATTGCTTCAACA rrnAB RibosomalRNA CTCTTGTCAGGCCG.GAATAACTCCCTATAATGCGCCACCACTG str Ribosomal protein TTCTTGACACCTT.TCGGCATCGCCCTAAAATTCGGCGTCG rpoA RNA polymerase TTCTTGCAAAGTTGGGTTGAGCTGGCTAGATTAGCCAGCCA TTGaca TAtAaT R

  5. N A Promoter recognition by sigma factors TTGaca TAtAaT R uvrB Repair DNA damage TTGTTGGCATAATTAAGTACGACGAGTAAAATTAC ATACCT recA DNArecombination CACTTGATACTGTA.TGAGCATACAGTATAATTGCTTCAACA rrnAB RibosomalRNA CTCTTGTCAGGCCG.GAATAACTCCCTATAATGCGCCACCACTG str Ribosomal protein TTCTTGACACCTT.TCGGCATCGCCCTAAAATTCGGCGTCG rpoA RNA polymerase TTCTTGCAAAGTTGGGTTGAGCTGGCTAGATTAGCCAGCCA Kp nifE nitrogenase accessory CTTCTGGAGCGCGAATTGCA TCTTCCCCCT Kp nifU nitrogenase accessory TCTCTGGTATCGCAATTGCT AGTTCGTTAT Kp nifB nitrogenase accessory CCTCTGGTACAGCATTTGCA GCAGGAAGGT Kp nifH nitrogenase CGGCTGGTATGTTCCCTGCACTTCTCTGCTG Kp nifM nitrogenase accessory TGGCTGGCCGGAAATTTGCA ATACAGGGAT Kp nifF nitrogenase accessory AACCTGGCACAGCCTTCGCA ATACCCCTGC Kp nifL nitrogenase regulat’n ATAAGGGCGCACGGTTTGCATGGTTATCACC glnA P2 glutamine synthetase AAGTTGGCACAGATTTCGCTTTATCTTTTTT CTGG-A TTGCA

  6. A A A A H H H H Sigma factors in sporulation Starvation (and other signals)Stage 0

  7. A A A A F F E E Sigma factors in sporulation H H Starvation (and other signals)Stage II/III

  8. A A A A E E H H F F H H Sigma factors in sporulation Starvation (and other signals)Stage 0

  9. A A A A E E F F F F F F E E Sigma factors in sporulation H H Starvation (and other signals)Stage II/III

  10. A A K K G G F F E E Sigma factors in sporulation Starvation (and other signals)Stage III Starvation (and other signals)Stage IV

  11. Sigma factors in sporulation Sporulation: regulated sigma factor cascade … but what regulates the sigma factors? • Not transcriptional control • What kind of post-transcriptional control? • How are events coordinated? Checkpoints?

  12. Sigma factors in sporulation Old gene namesNew gene namesProtein namesLocation rpoD sigA 37,A vegetative spo0H sigH H vegetative spoIIG sigE 37,E mother cell spoIIA sigF F forespore spoIIIC sigK K mother cell spoIIIG sigG G forespore

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