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Amino Transferases ( AST , ALT ) Colorimetric Methods

Amino Transferases ( AST , ALT ) Colorimetric Methods. 1) Retiman & Frankel Method 2) Retiman & Frankal Method equivalent to kinetics 3) Kinetic Method ( IFCC , DGKC , SCE , ECCLS ) 4) Dry Chemistry. Retiman & Frankel Method. L . Aspartate + α Ketoglutatate

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Amino Transferases ( AST , ALT ) Colorimetric Methods

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  1. Amino Transferases ( AST , ALT ) Colorimetric Methods 1) Retiman & Frankel Method 2) Retiman & Frankal Method equivalent to kinetics 3) Kinetic Method ( IFCC , DGKC , SCE , ECCLS ) 4) Dry Chemistry

  2. Retiman & Frankel Method L . Aspartate+αKetoglutatate Oxaloacetic+ L . Glutamate AST

  3. Kinetic Method ( IFCC ) Oxoglutarate+Aspartate Glutamate +Oxaloacetate Oxaloacetate+NADH + H+Malate + NAD + • Decrease in A340nm directly proportional to AST AST MDH

  4. Liner Colorimetric Method Reitman & Frankal

  5. Semi – Log Paper Colorimetric Method Reitman & Frankal

  6. Lab. Results of Transaminases for Different Methods

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