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Benzidine Test. BENZIDINE TEST. What is meant by benzidine test? is a test to detect the presence of blood at the scene of crime is a presumptive test to detect blood. What is Benzidine ?. Benzidine is a manufactured chemical does not occur naturally
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BENZIDINE TEST What is meant by benzidine test? is a test to detect the presence of blood at the scene of crime is a presumptive test to detect blood
What is Benzidine? • Benzidine is a manufactured chemical • does not occur naturally • It is a crystalline solid that may be grayish-yellow, white, or reddish-gray • Benzidine was used to produce dyes for cloth, paper, and leather. • It is no longer produced or used commerically in the U.S
Apparatus and Chemicals Required • Test Tube/ Filter Paper • Gloves • Droper • Benzidinedihydrochloride • Glacial acetic acid • hydrogen peroxide
Benzidine Test Procedure and Observations • Take 3 ml of fresh benzidine reagent in a test tube. • Add 2 ml of sample in it. • And also add 1 ml of 3% hydrogen peroxide in it. • Observations: A blue or green colour will develop if haemoglobin is present in the sample. This mean blood is present
Precautions to be taken • Before start performing experiment make sure to wash the apparatus . • As always, carefully handle all the chemicals . • Avoid sample touching with hands while doing the experiment. • Use test tube holder for holding test tube. • The test tube used should be clean neatly and free of any dirt and chemicals because we will not get the proper result then.
Strengths of Benzidrine test • An easy test • Can be performed by any person /IO • Save valuable initial time during investigation
Drawbacks of Benzidine Test • It Is a presumptive test only • Benzidine is a carcinogenic substance