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Comprehensive Analysis of Abl and Bcr-Abl Interactions Using GST-Myc Fusion Proteins

Explore Abl and Bcr-Abl interactions by investigating GST-Myc fusion proteins targeting specific domains, enabling a detailed understanding of their functions and regulatory mechanisms.

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Comprehensive Analysis of Abl and Bcr-Abl Interactions Using GST-Myc Fusion Proteins

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  1. + - + GST-Myc 1-262 - + - GST - + + Abl a b Flag-Myc 5YF - - - + GST Myc 1-262 - + - + Flag-Myc WT P-Tyr Abl - - + + Abl P-Tyr Total: IP:Flag Myc GST Myc 1-262 GST Total: Abl GST c d - - - - + Myc T58A - - + + Bcr-Abl - - - + - Myc 5YF - - - + Flag-Myc Y74F Myc WT - - - + - + + - + Flag-Myc WT Bcr-Abl - - + + + P-Tyr IP Myc pY74 Flag Myc Total Flag Bcr-Abl Total Vinculin Bcr-Abl Sanchez-Arévalo et al. Fig. S2

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