Mouse Genetics Interest Group :

1. Mouse Genetics Interest Group : AGENDA Mouse model organization -planning -information Rat colony Mouse Genetics Interest Group 04 27 2011

2. Ongoing organization : MOUSE production Notebook PROCEDURE PUPS caHSF1 rtTA X Tg/Tg Tg/+ INFORMATION INVENTORY on the server BREEDING SHEET In FOLDER Mouse Genetics Interest Group 04 27 2011

3. Ongoing organization : MOUSE UTILIZATION INFORMATION INVENTORY on the server BREEDING SHEET In FOLDER WHO ? WHEN ? FOR WHICH EXPERIMENT ? Mouse Genetics Interest Group 04 27 2011

4. Ongoing colony – Number of cages / project = TOTAL 300 cages 110 84 60 47 Number of cages Mouse Genetics Interest Group 04 27 2011

5. but …. do you have what you need to perform your experiments ….. Mouse Genetics Interest Group 04 27 2011

6. Planning … information needed to prepare mouse planning : 1- what is written in the grant/ project ? 2- define experimental groups 3- number of animals / groups 4- age 5- gender An idea TODAY An experiment ….. 1 month + x month old experimental age Mouse Genetics Interest Group 04 27 2011

7. Planning … • How to set up the breeding to get the expected number : • -breeding efficiency of the mouse strain : • - from 6 to 11 pups/female • -Mendelian laws • genetic trait • sex ratio Mouse Genetics Interest Group 04 27 2011

8. Planning … Exple1 : R120G B6J background : 10 males Tg/+ and 10 siblings +/+ (6mo old) 20 males  40 pups  Minimum 7/ better 10 females in breeding : 10 cages cost : 10 cages / 30 days = 186 USD Min 6 cages / 180 days = 670 USD TOTAL = 856 /20 males  1 exp. Male : about 45 USD Mouse Genetics Interest Group 04 27 2011

9. Planning … Exple2 : caHSF1 FVB background : 10 males Tg/+ and 10 siblings +/+ (2mo old) 20 males  40 pups  Minimum 5/ better 7 females in breeding : 7 cages cost : 7 cages / 30 days = 130 USD Max 10 cages / 60 days = 372 USD TOTAL : 502 /20 males  1 exp. Male : about 25 USD Mouse Genetics Interest Group 04 27 2011

10. What we can do for your mouse projects What you should do for your mouse projects -know your model, strain, genetic modifications, litterature -read the animal protocol and prepare the necessary update/amendment -plan your experiments -animals -provide the information about the required animals to be generated - provide the information if you go to the colony and take the animals by yourself -regenotype your exp. animals after use -ALWAYS identify the exp. animals (ID from the colny used in each experiment -order any required animal -set up the breeding -take care of the breeders, pups -wean -tail -genotype -inventory -deliver the exp. animals Mouse Genetics Interest Group 04 27 2011

11. Identification of the animals ∧ Ear PUNCH : 1R ΩΩ ° ΩΩ 1L ° ΩΩ 1R1L ° ° ΩΩ 2R ° ° ΩΩ 2L ° ° ΩΩ ° ° ° ° Ear TAG : only 2 lines Mouse Genetics Interest Group 04 27 2011

12. PARENTS Female (ID) x Male (ID) Genotype Genotype DOM DOB1 DOB2 Breeding Line name DOB PARENTS Female (ID) x Male (ID) Genotype Genotype Tx 1 (1R) Genotype Tx2 Weaned pups Line name

13. RAT colony Mouse Genetics Interest Group 04 27 2011

14. Inventory Mouse Lines (9 & related crosses) Mouse Genetics Interest Group 02 23 2011

15. Cage card information GENOTYPE – (genome) Transgenic : +/+ : wild type Tg/+ : hemizygote ( heterozygote) Tg/Tg : homozygote KO (constitutive) : +/+ : wild type -/+ : hemizygote (heterozygote) -/- : homozygote –null KO (conditional) : F/F : floxed del/F: hemizygote del/del: homozygote - null Mouse Genetics Interest Group 01 26 2011

16. Creation LINE: molecular biology/mouse Establishment : breeding history Maintenance / Experiments: breeding, animal ID, genotype, record breeding sheet (breeding inventory) mouse inventory : one ! per line genotype animal : 17 - 30 days MAX regenotype experimental animal after EUTH indicate ID for each experimental animal in experiment protocol