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MES-4

MES-4 . Chromatin Regulation in the C. elegans Germ Line. XX. soma. both Xs are reduced ~50%. XO. Dosage compensation in worms:. germline. Xs are not transcribed. How chromatin is organized…. H3. methylated Lys 20 silencing. The Histone Code. N. N. H4. H3. N. N. H4. N. N.

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MES-4

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  1. MES-4 Chromatin Regulation in the C. elegans Germ Line

  2. XX soma both Xs are reduced ~50% XO Dosage compensation in worms: germline Xs are not transcribed

  3. How chromatin is organized…

  4. H3 methylated Lys 20 silencing The Histone Code N N H4 H3 N N H4 N N acetylated Lys 8, 12, 16 activating methylated Lys 27 silencing methylated Lys 9 silencing methylated Lys 4, 36 activating

  5. The nucleosome. H2A Get better resolution! H3 H2B H4 Ref. Kornberg and Lorch, Cell 1999

  6. MES-4 + DNA DNA alone MES-4 binds to just 5 of the 6 chromosomes. maternal pronucleus paternal pronucleus

  7. H3-Lys36 di-methylation is on Xs in early embryos. MES-4 is required for H3-Lys36 di-methylation. 1-cell WT 2mK36 WT mes-4 mes-4

  8. Chromatography

  9. Sucrose Gradients

  10. Gel Electrophoresis

  11. Antibody Use in Detection of Target Protein

  12. MES-4 and its homologs NSD1 catalyzes H3 Lys36 and H4 Lys20 methylation in vitro. (Raysam et al., EMBO J 2003)

  13. C. elegans (MES-4) and C. briggsae (CBG60706) Homologs

  14. MES-4 and CBG60706

  15. CB60706 and MES-4 Introns and Exons

  16. Nucleotide Alignment

  17. BLAST Amino Acid Alignment Under Default Settings

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