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Micropipettes

Micropipettes. Measuring volumes which are what we scientists call “very, very small”. MICROPIPETTE RULES. Don’t set the pipettes past their intended range (high or low), even though you can. ALWAYS return the dial to the highest setting before putting the pipette away.

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Micropipettes

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  1. Micropipettes Measuring volumes which are what we scientists call “very, very small”

  2. MICROPIPETTE RULES • Don’t set the pipettes past their intended range (high or low), even though you can. • ALWAYS return the dial to the highest setting before putting the pipette away. • ALWAYS return the pipette to its correct storage bin

  3. High Range: 100-1000 µL Use large blue or white tips Digits from top to bottom represent: 1000’s 100’s 10’s The 1’s are indicated by the tiny white tick marks in the dial with the 10’s. Marks are in increments of 2 µL This pipette is set to 1000 µL

  4. This pipette is set to 998 µL

  5. This pipette is set to 990 µL

  6. Mid-range: 5.0-50.0 µL10.0-100.0 µL, 20.0-200.0 µL Range is printed on the button or on the side Use medium sized yellow or white tips

  7. 10.0-100.0 µL This pipette is set to its maximum: 100.0 µL Digits represent: 100’s 10’s 1’s Tick marks represent 0.1’s in increments of 0.2 µL

  8. Low Range: 0.50-10.00 µL 2.00-20.00 µL Digits represent: 10’s 1’s 0.1’s Tick marks are 0.01’s in increments of 0.02 µL Use small white tips

  9. Rainin 0.50-10.00 µL pipettes use a unique tip Clear tip has an extra wide mouth that surrounds and goes over the barrel and eject bar. These tips can ONLY be used with the Rainin 0.5-10

  10. Feel the stops before you begin taking up or dispensing any liquid. 1st stop: Exact volume to which the pipette is set. Press to this stop before immersing pipette tip in the desired solution. 2nd stop: Use when dispensing solution. Pushes an extra volume of air to dislodge any solution adhering to the tip.

  11. How to pipette Great summary

  12. Extract and dispense SLOWLY. • When dispensing, touch the tip • to the side or bottom of the tube, • or if there is liquid already in the • tube, touch the tip to the liquid. • When taking up or dispensing • liquid, don’t put the tip any deeper • into the liquid than necessary.

  13. NEVER hold pipette so that the tip is higher than the handle or liquid leaks into the barrel (BAD!) • Always hold pipettes vertically • when in use. • Check to be sure the tip is on securely. • Only open the tip box when getting a tip – DON’T LEAVE IT OPEN!

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