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The Cellular Level of Organization –Part B

The Cellular Level of Organization –Part B. Cytoplasm. Cytosol Semifluid, mostly water Protein, carbohydrates, lipids, and inorganic substances (ions) Many important metabolic reactions take place here Cytoplasm is the cytosol plus the organelles. Organelles.

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The Cellular Level of Organization –Part B

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  1. The Cellular Level of Organization –Part B

  2. Cytoplasm • Cytosol • Semifluid, mostly water • Protein, carbohydrates, lipids, and inorganic substances (ions) • Many important metabolic reactions take place here • Cytoplasm is the cytosol plus the organelles

  3. Organelles “little organs” – have characteristic appearance and have specialized functions in cell growth, maintenance, repair and control. Number and types of organelle vary with cell. Two basic types: nonmembranous membranous

  4. Ribosomes • Made of ribosomal RNA and protein • Free and associated with the endoplasmic reticulum ( fixed ribosomes) • Site of protein synthesis • Nonmembranous, Temporary structures made of large and small subunits

  5. Proteasomes • Hollow cylinders which contain protein- digesting enzymes ( proteases) • Remove and recycle damaged or denatured proteins • Also play a role in the immune response

  6. Endoplasmic reticulum (ER) Endo – within Plasm – cytoplasm Reticulum - network Can be rough or smooth Rough ER has ribosomes, makes proteins for export outside of cell

  7. Smooth Endoplasmic Reticulum • Synthesis of phospholipids and cholesterol • Synthesis of steroid hormones • Synthesis and storage of triglycerides • Synthesis and storage of glycogen • Storage of ions ( e.g. Ca++ in muscle) • Detoxification and inactivation of drugs

  8. Gogli apparatus (body, complex) Made of flattened sacs called cisternae cis, medial, trans -Process, sort and deliver proteins and lipids to the plasma membrane-forms vesicles and lysosomes.-forms new plasma membrane The “UPS” of the cell

  9. Vesicles • Membrane bound sacs • Formed during endocytosis or by golgi apparatus • Transport • Renew membrane – membrane flow

  10. Lysosomes The cell’s “stomach” – vesicles that contain digestive enzymes. Primary lysosomes contain inactive enzymes work at low pH Found in large numbers in white blood cells Intracellular digestion ( phagocytosis etc.)worn-out organelles ( autophagy)digestion of cell contents (autolysis) Tay-Sachs disease

  11. Peroxisomes • Smaller than lysosomes • Enzymes use molecular oxygen to oxidize substances • Reactions produce Hydrogen Peroxide- H2O2 • Free radicals

  12. Mitochondria Mitochondrion – singular Two membranes – inner folds called cristae. Main function is the use of oxygen to produce ATP – cellular (aerobic) respiration These are the “power plants” of the cell. Have their own DNA and RNA

  13. Centrosomes and Centrioles Centrioles are 2 cylinders of tubules arranged at right angles.9 + 0 array Form the microtubules of the mitotic spindle during cell division, and also make up a part of cilia and flagella Heart of the cytoskeleton

  14. Flagella and cilia • Cilia – “eyelashes” cilium • 9+2 array • Anchored to basal body

  15. Flagella

  16. Cytoskeleton Microfilaments, intermediate filaments and microtubules Give the cell shape, and allow it to move – the Musculoskeletal system of the cell. Myofilaments are made of the protein actin Myotubules are made of the protein tubulin

  17. Inclusions Usually contain chemical substances produced by the cell, these are temporary structures that are not surrounded by a membrane. Melanin, glycogen, triglycerides ribosomes

  18. Nucleus • Double membrane called the nuclear envelope • Nucleoplasm • Chromatin granules – unwound DNA • Nucleoli – puts RNA and protein together to make ribosomes • Nucleus is essential for cell survival

  19. The Cell Cycle • Nuclear division (mitosis or meiosis) • Cytoplasmic division – cytokinesis • Mitosis is somatic (body) cell division • Meiosis is reproductive cell division

  20. Mitosis Homologous chromosomes Cell cycle – from one cell division to the next Interphase – “resting phase” G1 – Gap1 – growth phase S – Synthesis – replication of DNA G2 – Gap 2

  21. Nuclear division – mitosis or karyokinesis Prophase Metaphase Anaphase Telophase PMAT

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