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Il ruolo del PTP in apoptosi/necrosi. Some characteristics common to several diseases: . Oxidative damage Changes in Calcium homeostasis Loss of energy production. Cell death. Tissue and organ dysfunction. Disease. c. c. c. c. c. c. The Mitochondrial connection.
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Some characteristics common to several diseases: Oxidative damage Changes in Calcium homeostasis Loss of energy production Cell death Tissue and organ dysfunction Disease
c c c c c c The Mitochondrial connection. Mitochondria are key integrators of cellular signals and stress: make life and death decisions IMM OMM Bax/Bcl2 mPTP Swelling, rupture Release of proapoptotic factors Apoptosis and necrosis are facilitated at the level of the mitochondria by the opening of large pores (excluding extrinsic apoptotic pathway)
ANT: Adenine nucleotide transporter, VDAC: voltage dependent anion channel, HK: Hexokinase; CyD: Cyclophilin D, CK: Creatine kinase, PBR: Periferal benzodiazepin receptor The Mitochondrial Permeability Transition. • Mitochondrial Permeability transition (mPT) characterized by: • Loss of inner membrane potential; • Release of Cytochrome C; • Cell death by apoptosis or necrosis; • Cessation of mitochondrial respiration; • Release of mitochondrial Ca 2+; • Mitochondrial swelling (rupture) • The mPT can be caused by several agents/mechanisms (e.g. ROS, Ca2+ overload); • The mPT, and as a consequence, mitochondrial dysfunction, is associated with the onset and progression of several diseases.
Recentisviluppinellaidentificazionedellecomponenti del PTP La ATP sintetasi, maggioreproduttorecellulare di ATP nellacellula, èstatarecentementeproposta come l’unicocomponentemolecolarenecessario per la formazione del PTP: questodatoèancora da confermare in manieradefinitiva, ma enfatizza la funzionedualedeicomponentidella catena respiratorianelcontrollo di apoptosi e necrosi (cheritroveremo con ilcitocromo C)
Consequences of mPTP opening stimulus mPTP opening loss of membrane potential/energy production solutes across mPTP enter mitochondria mitochondria swell and outer membrane ruptures mitochondria swell and outer membrane rupture Ca++ and protein release (eg. Cytochrome c) Cell death
The mPTP and Disease. • Opening of mPTP has been implicated in the aetiology and progression of several diseases including : • Neurodegenerative diseases (Parkinsons, Alzheimers, MS, Huntingtons, ALS) • Ischemia / reperfusion injury (AMI, Stroke, organ transplantation) • Dystrophies (Bethlem, Ullrich, Duchene) • Diabetic Complications (retinopathy, nephropathy) • Oxidative damage, irregular calcium signalling and mitochondrial dysfunction is common to all of these diseases
Take a closer look at Ischemia / reperfusion injury (from a Mitochondrial standpoint only)
Lethal reperfusion Injury. • Heart ischemia leads to infarct size of > 70%. • Reperfusion of tissue can reduce infarct size. • Paradoxically reperfusion also leads to tissue death and contributes to infarct size (LRI). • Methods to reduce lethal reperfusion injury should have clinical benefit
PTP in Ischemia / Reperfusion Injury What’s happening in the the cell and mitochondria: Normal conditions Calcium enters mitochondria via the Calcium Uniporter (not saturated at High [Ca2+]) Ca2+ Ca2+ Uniporter Cell Ca2+ Mitochondria Na/Ca2+ Antiporter Calcium is pumped out of the mitochondria via the Sodium / Calcium Antiporter (saturated at High [Ca2+]) Under conditions of high cytoplasmic [Ca2+] the mitochondria can overload with calcium and this can cause the mPT.
Reoxygenation conditions Reoxygenation conditions Ischemic conditions Ischemic conditions Na+/Ca2+ Antiporter Ca2+ Na+ Ca2+ Ca2+ Ca2+ Ca2+ Ca2+ mPTP CytC Ca2+ Ca2+ Uniporter Na+ Ca2+ Na+ Ca2+ Ca2+ Ca2+ Cell Death Necrosis Apoptosis Na+ Na+ pH [H+] Ca2+ Na+ H+ Ca2+ Cell Cell Cell Cell Cell Ca2+ CytC Ca2+ Ca2+ Ca2+ Ca2+ Na+ Ca2+ Ca2+ Mitochondria Mitochondria Mitochondria Mitochondria Mitochondria Mitochondria CytC Ca2+ H+ Na+ H+ Na+ H+ Na/Ca2+ Antiporter H+ Na+/H+ Antiporter H+ Na+ H+ Restart to respiration. Large burst of ROS production. Mitochondria load with calcium via uniporter and saturate the Na+/Ca2+ antiporter. Calcium overload and oxidative damage from ROS production induce opening of the mPTP and mitochondrial dysfunction. Cell death via necrosis/apoptosis depending on damage. Loss of ATP production via respiration; increase in lactic acid and a drop in cellular pH. High intracellular [Na+] causes reversal of the Na+/Ca2+ Antiporter and the cell loads with Ca2+. To counter the high [H+] the cell uses the Na+/H+ Antiporter. Due to lack of ATP the Na+ can not be pumped out and the cell loads with Sodium. PTP in Ischemia / Reperfusion Injury Ischemic conditions
I topi KO hanno un difetto nell’apertura del PTP, ma sono normali Apertura del PTP in mitocondri isolati
Cellule PTP -/- sono resistenti alla morte cellulare indotta da ROS (H2O2)
I topi KO sono resistenti al danno cardiaco indotto da ischemia-riperfusione
New cardioprotective strategies. • mPTP inhibitors could be a promising strategy for lethal reperfusion injury. From Yellon and Hausenloy (2007 NEJM)
Inibitori del PTP (CsA) sono anche efficaci nella riduzione del danno post-ischemico dell’Uomo Marker sierico Area infarto (NMR)
The mPTP is involved in the pathogenesis of several aging-associated diseases • Studies using CsA and Ppif-/- mice (cyD null) have highlighted a potential role for the mPTP in the progression of several diseases. Dystrophies Neurodegenerative Disease Reperfusion Injury Myocardial infarction ALS Alzheimer’s Disease Duchenne MD Piot et al 2008 Millay et al, 2008 Du et al, 2009 Keep et al, 2001 Ullrich / Belthem Stroke Ppif -/- mice and CsA Korde et al, 2007 Palma et al, 2009 Other Coronary Artery Bypass Graft Heart Disease Transplantation and Surgery Diabetic Retinopathy Hyperglycemia Doxorubicin toxicity Platelet activation Traumatic brain injury Non-alcoholic fatty liver disease
L’identificazione di nuovifarmaciinibitoridell’apertura del PTP
A large network of industry/academia interactions Hamburg Oxford:EVOTEC Lyon:M Ovize Padua: P Bernardi F Di Lisa Milan:CONGENIA PG Pelicci R Latini Nikem NMS
Ca2+ 150 μM 1 mM CsA Absorbance (A 540 nm) Control 0 5 10 Time (mins) Innovative HTS • Organelle based • Requiring the use of animals->liver mitochondria unprecedented at this scale • A high pulse of Ca2+ (ca. 150 mM) given to purified mitochondria will cause mPTP opening and induce mitochondrial swelling (DA540nm). • A Ca2+-induced swelling assay was developed suitable for HTS • Fresh Rat liver mitochondria were prepared daily and used in this assay to screen >300,000 compounds • Counterscreens were run to elimate compounds that interfere with mitochondrial respiration. • Several chemical classes were identified
Adaptation of mitochondrial assays to 1536 well format • Parameters to optimize: • density / fitness of mitochondria • concentration of stimulus (Ca++) • assay sensitivity (CysA) • reagents volumes / order of addition • reagents incubation times • reagents dispensing devices • readout kinetics / stability of assay signal • DMSO-sensitivity • reader settings (i.e. OD filter sets) Results of time-course experiment in 1536 well plates: 4 Fitzones Z‘: 0,74 / 0,76 / 0,70 / 0,61 Mitochondria: 1 mg/ml Calcium: 300 µM Incubation: 10 min Cyclosporin A: 1 µM
HTS-Hardware in Operations 2 EVOscreen® Mark II: biochemical assays at 1 µL level 1 EVOscreen® Mark III: biochemical & cellular assays at 1-10 µL level
GNX-A Untreated Absorbance (A 540 nm) Ca2+ 150μM, pH 7.4 Treated Time (mins) PTPi block mPTP openingby several stimuli • Class 6 compounds inhibit the mPT induced by: • Calcium overload • Oxidative damage • Protein crosslinkers • Respiration uncouplers GNX-A GNX-A GNX-A Untreated Untreated Untreated A 540 nm A 540 nm A 540 nm Treated Treated Treated Time (mins) Time (mins) Time (mins) 50nM FCCP 40μM Ca2+, pH 7.4, 100μM Menadione 40μM Ca2+, pH 7.4, 300μM Diamide, 40μM Ca2+, pH 7.2, Assay performed on prepared mouse hepatic mitochondria
100 90 80 70 60 50 40 100.0 30 90.0 20 80.0 10 70.0 0 60.0 -10 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 50.0 -20 -30 40.0 30.0 20.0 10.0 0.0 0 5 10 15 20 25 100 90 80 70 60 100.0 50 90.0 40 80.0 70.0 30 60.0 20 50.0 10 40.0 0 30.0 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 -10 20.0 -20 10.0 0.0 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 -10.0 PTPi do not have significant effects on mitochondrial respiration State 3 inhibition (with ADP) State 3 inhibition (with ADP) % max inhibition % max inhibition [GNX-C] (mM) [GNX-B] (mM) State 4 inhibition State 4 inhibition % max inhibition % max inhibition [GNX-C] (mM) [GNX-B] (mM)
Control GNX-B (0.1mM) CsA (1μM) GNX-B (1.0mM) GNX-B (5.0mM) Ca2+ Release mPTP open Ca2+ Pulses (10 mM) 350 Extra-mitochondrial Calcium fluorescence 300 Control 250 200 CRC (mM Ca2+) 150 40 mM 120 mM 180 mM 250 mM 380 mM 100 50 Time (min) 0 0.1 mM 0.5 mM 1.0 mM 5.0 mM 0.1 mM 0.5 mM 1.0 mM 5.0 mM GNX C CsA The identified PTP inhibitors are >> than CsA • Class 6 compounds are able to increase the CRC (by mPTP inhibition) several fold over that of unprotected mitochondria. • Class 6 compounds increase the CRC significantly over that maximally attainable with CsA. The Calcium Retention Capacity (CRC) of purified mouse liver mitochondria is determined by measuring the point at which pulse-loaded calcium is released from the mitochondria. After the addition of a pulse of calcium (10 mM) the extramitochondrial fluorescence increases. As the mitochondria take-up the calcium the fluorescence signal decreases. Calcium is continually loaded into the mitochondria until there is a sudden, large, increase in calcium fluorescence which indicates complete release of the stored calcium due to opening of the mPTP. Inhibition of the mPTP, with Cyclosporin A or our proprietary inhibitors can increase the capacity of mitochondria to retain calcium and thus protect the mitochondria from calcium overload in stress situations. Extramitochondrial calcium is measured by the fluorescence of calcium green. Note, increasing the concentration of CsA does not increase the CRC due to saturation of its target (Cyclophilin D) and is one of its limitations.
PTP inhibitors for Acute Myocardial Infarction The highway to proof-of-concept: unmet medical need strong evidence for involvment of the mPTP linear path for the design of a clinical study
. . . vehicle A-B . 0.6 . . GNX -B . No protection . . . . CsA . 0.5 Protection . 0.4 . . Arae Necrotic (g) . 0.3 . . 0.2 . . . 0.1 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 Area at Risk (g) PTP inhibitors are cardioprotective in animal models New Zealand White Rabbits were subjected to Left Anterior Descending (LAD) coronary artery occlusion for 30 mins followed by 4 hrs of reperfusion. Area at Risk and infarcted area (area necrotic) was determined by Evans blue and TTC staining. GNX-B (15 mg /kg in 3 mls 40% PEG 400; 20% DMSO) and CsA (10 mg/kg in sandimune) were administered by i.v. bolus 5 mins prior to reperfusion. Note, as with CsA in the clinical proof of concept trial, there is greater protection when the area at risk is larger. (In collaboration with Prof Ovize, Ospice di Lyon)
Un ruoloinaspettato per p53 nell’induzione di necrosi Dopo stress, p53 sitrovalocalizzatainsieme con la ciclofilina-D aimitocondri
P53 èrichiesta per l’apertura del PTP durante la necrosi da stress ossidativo Vienemisuratal’apertura del PTP
Lo stress ossidativo induce necrosi e non apoptosichedipende da p53
Considerazioni • P53 è un regolatorefondamentaledell’apoptosi, ma sembragiocare un ruoloaltrettantorilevantenellamortecellulare da necrosidopo stress ossidativo • P53 è “good” e “bad”: in questocasopotrebbeesserecoinvoltonellapatogenesi di moltemalattie • Il coinvolgimento di p53 èun’altradimostrazionecheanche la necrosièsottoposta (almeno in alcunicasi) a un livello di regolazioneimpensabilefino a poco tempo fa