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CEREBROSPINAL FLUID

CEREBROSPINAL FLUID. CSF FUNCTIONS. 1. Supply Nutrients 2. Remove Metabolic Waste 3. Protect and Cushion Brain and Spinal Chord. CSF Volume. Adults 140 to 170 mL Neonates 10 to 60 mL Located in the subarachnoid space between the arachnoid matter and pia matter.

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CEREBROSPINAL FLUID

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  1. CEREBROSPINAL FLUID

  2. CSF FUNCTIONS • 1. Supply Nutrients • 2. Remove Metabolic Waste • 3. Protect and Cushion Brain and Spinal Chord

  3. CSF Volume • Adults 140 to 170 mL • Neonates 10 to 60 mL • Located in the subarachnoid space between the arachnoid matter and pia matter. • 20 mL produced per hour

  4. CSF Production • Produced in the Choroid Plexus of brain • DOES NOT resemble ultrafiltrate of plasma • Large molecules are restricted • Blood Brain Barrier

  5. CSF Collection • Lumbar puncture • Tube 1 Chemistry and Serological testingFreeze • Tube 2 Microbiology RT • Tube 3 Hematology Refrigerate • STAT • DO NOT Discard Supernatant

  6. CSF Appearance • Clear and Colorless – Normal • Cloudy or Turbid Protein, Cells Lipids • Xanthochromic pink, orange or yellow due to RBC degradation products

  7. Traumatic Tap • Uneven distribution of Blood • Clot Formation • Xanthochromic Supernatant

  8. Chemistry Tests • Protein 15 – 45 mg/dL • Protein electrophoresis • Glucose 2/3 of blood glucose • Lactate • Glutamine 8 – 18 mgdL • Enzymes LD CK-BB , 17 mg/dL

  9. Chemistry Tests • Protein 15 – 45 mg/dL • Methods Turbidometric or Dye Binding • Turbidometric TCA or SSA • Dye Binding Coomassi Brilliant Blue • Electrophoresis

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