1 / 4

Susceptibility Testing

Susceptibility Testing. Kirby-Bauer by Jessica Davies. What is it??. It determines the sensitivity or resistance of pathogenic aerobic and facultative anaerobic bacteria to various antimicrobial compounds Helps assist a physician in selecting treatment options for his or her patients.

pete
Download Presentation

Susceptibility Testing

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Susceptibility Testing Kirby-Bauerby Jessica Davies

  2. What is it?? • It determines the sensitivity or resistance of pathogenic aerobic and facultative anaerobic bacteria to various antimicrobial compounds • Helps assist a physician in selecting treatment options for his or her patients

  3. How is it performed?? • 1.      Sample colony with a straight wire. • 2.      Prepare a suspension in 2.5 ml normal saline. • 3.      Inoculate a pre-dried agar plate. • 4.      Distribute the inoculum by rocking. • 5.      Remove excess inoculum. • 6.      Dry the plate at room temperature. • 7.      Load the plate with antibiotic discs. • 8.      Incubate for 18 hours. • 9.      Measure the annular radii. • 10.   Interpret zone sizes.

  4. How do you interprete it?? • Simplest explanation is the larger the area of bacteria free space around the antibiotic discs, the less resistance the bug has against that antibiotic. Therefore it is the better antibiotic to use for treatment.

More Related