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THANATOCHEMISTRY

THANATOCHEMISTRY. CRYOGENY. Cryogenics: A How-To Manual. 1) Remove head 2) Immerse head in hypertonic glycerol bath 3) Place head in liquid nitrogen until ??? 4) Place head in distilled water 5) Enjoy!. Why doesn’t cryogenics work?. TONICITY.

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THANATOCHEMISTRY

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  1. THANATOCHEMISTRY

  2. CRYOGENY

  3. Cryogenics: A How-To Manual • 1) Remove head • 2) Immerse head in hypertonic glycerol bath • 3) Place head in liquid nitrogen until ??? • 4) Place head in distilled water • 5) Enjoy!

  4. Why doesn’t cryogenics work?

  5. TONICITY • Def: Relative measure of dissolved particles (solute) in the solution surrounding the cell membrane • Measurement is always compared to interior/cytoplasm of cell • Water does not have a tonic classification as it is invariably the solvent

  6. PLASMOLYSIS • DEF: Loss of water and turgor due to placement of cell in HYPERTONIC environment • Water leaves cell via osmosis, causing vacuole to shrink, or cytoplasm to lose volume (crenation)

  7. CYTOLYSIS • Def: Cells placed in HYPOTONIC environments may undergo cytolysis/cell rupture as water enters the cell • Loss of the lipid bilayer ultimate causes cell death

  8. TURGOR • In plants, the cell wall resists cytolysis in hypotonic environments • This turgor pressure allows the plant to resist gravity

  9. Lab: Semipermeable Membranes and Osmosis • Purpose: To analyze the movement of solutes and water across a selectively permeable membrane • Method: Tracking movement of solutes and water achieved by use of organic indicators and qualitative description of turgor (rigidity of fluid filled container)

  10. Dialysis • Loss of kidney membrane permeability due to disease or damage requires dialysis • Wastes are removed from body by passing blood through an artificial cell membrane

  11. Protocol #1 • Fill beaker 2/3rds full with tap water • Add Lugol’s solution until mixture is amber

  12. Protocol #2 • Open wet dialysis tube using fingers and glass rod. Tie one end off with string • Using seral pipettes fill tube with starch and glucose solutions • Tie off open end. Do NOT leave space for air • Trim the strings and excess tubing

  13. Protocol #3 • Immerse “cell” in iodine-water bath • Allow to sit for 20 minutes while doing Protocol #4 • After 20 minutes, qualitatively assess color of bath, cell and turgidity of cell • Retest water with Tes-Tape to check for presence of Glucose

  14. Protocol #4 • Observe Elodea cells in fresh water (pre-made slide) and sketch • Make a 2nd slide using one Elodea leaf and a drop of 6% NaCl solution • Sketch the 2nd slide, noting any changes between the fresh water and salt water

  15. Lab: Semipermeable Membranes and Osmosis • Purpose: To analyze the movement of solutes and water across a selectively permeable membrane • Method: Tracking movement of solutes and water achieved by use of organic indicators and qualitative description of turgor (rigidity of fluid filled container)

  16. Protocol #1 • Fill beaker 2/3rds full with tap water • Add Lugol’s solution until mixture is amber • Test water with Tes-Tape to determine if glucose is present in the bath

  17. Protocol #2 • Open wet dialysis tube using fingers and glass rod. Tie one end off with string • Using seral pipettes fill tube with starch and glucose solutions • Tie off open end. Do NOT leave space for air • Trim the strings and excess tubing

  18. Protocol #3 • Immerse “cell” in iodine-water bath • Allow to sit for 20 minutes while doing Protocol #4 • After 20 minutes, qualitatively assess color of bath, cell and turgidity of cell • Retest water with Tes-Tape to check for presence of Glucose

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