指頭血による生体酸化包括的評価法と糖質摂取惹起酸化ストレスのビタミン C による抑制塩入麻由 1 , 井上葉子 1,2 , 矢部えん 1

2-IV-23 指頭血による生体酸化包括的評価法と糖質摂取惹起酸化ストレスのビタミンCによる抑制塩入麻由1, 井上葉子 1,2,矢部えん 1 長浦亜希子1, ○薗田勝1 1共立女子大・家政・食物栄養 2愛国短大・家政 VSOJ (2008) 2-IV-23 Sonoda-01

PKC p22 gp91 p47 Rac p67 Oxidative Stress Induced by Glucose-Flux Glucose GLUT p p NOX Gluc 6-P NADPH Pentose-P-pathway ROS ☆ Zhang D. & Gutterman D. Am J Physiol Heart Circ Physiol (2008) 292, 2023-2031 ☆Sies H. et al. J Nutr (2005) 135, 969-972☆ Ceriello A. Diabetes(2005) 54, 1-7☆Dandona P. et al. Am J Clin Nutr (2004) 80, 51-57☆Nishikawa T. et al. Nature (2000) 404, 787-790☆Ghanim H. et al. Diabetes Care (2007) 30, 1406-1411☆Newsholme P. et al. J. Physiol (2007) 583.1, 9-24 VSOJ (2008) 2-IV-23 Sonoda-02

O 2 Oxidative Stress Induced by Glucose-Flux Glucose GLUT AGE Antioxidants Gluc 6-P Polyol Pathway ROS Pyruvate Cu, Zn-SOD NOS Uncoupling Glutathione-POD Glutathione-Red ETC Mt. Mn-SOD UCP VSOJ (2008) 2-IV-23 Sonoda-03

100 %-Hemolysis 50 hν 0 2 20 40 60 Propagation (min) 1 1 O O 2 2 Evaluation Method for The Redox Balance Whole Blood Photosensitization Initiation Reaction Post-Illumination Propagation Reaction Hemolysis Profile VSOJ (2008) 2-IV-23 Sonoda-04

100 120 min %-Hemolysis 60 min 50 30 min Fasted 0 2 2 20 40 20 40 60 60 Propagation (min) Propagation (min) 1 1 O O 2 2 The Redox Balance < Glucose 75g > MS : age 59, male MS : age 22, female VSOJ (2008) 2-IV-23 Sonoda-05

100 Fasted %-Hemolysis 50 30, 60, 120 min later 0 2 20 40 60 2 20 40 60 Propagation (min) Propagation (min) 1 1 O O 2 2 The Redox Balance < Ascorbate 600mg > MS : age 59, male MS : age 22, female VSOJ (2008) 2-IV-23 Sonoda-06

100 Sucrose : 30min Sucrose : 30min Fasted %-Hemolysis 50 Ascorbate : 30min Fasted 0 2 20 40 2 20 40 60 60 Propagation (min) Propagation (min) 1 1 O O 2 2 The Redox Balance < Sucrose 20g > < Asc & Suc > MS : age 22, female VSOJ (2008) 2-IV-23 Sonoda-07

100 Boiled Rice : 120min Boiled Rice : 60min Fasted %-Hemolysis 50 0 2 20 40 2 20 40 60 60 Propagation (min) Propagation (min) 1 1 O O 2 2 The Redox Balance < Boiled Rice 360g > MK : age 21, female EA : age 21, female VSOJ (2008) 2-IV-23 Sonoda-08

100 Boiled Rice 120min Boiled Rice : 60min Ascorbate : 30min %-Hemolysis Fasted 50 0 2 20 40 2 60 20 40 60 Propagation (min) Propagation (min) 1 1 O O 2 2 The Redox Balance < Boiled Rice 360g & Asc > MS : age 22, female Asc － Asc ＋ VSOJ (2008) 2-IV-23 Sonoda-09

100 %-Hemolysis 50 0 2 20 40 60 Propagation (min) Arbitrary Units for The Redox Balance Regression Slope VSOJ (2008) 2-IV-23 Sonoda-10

Sucrose : 120min Sucrose : 60min Fasted 360g (134g) 180g (67g) 20g 3.0 Boiled Rice : 120min Boiled Rice : 60min Fasted 1.5 Arbitrary Units 0 TK SY YK AE YH MK MK AE SY Redox Balance Changes by Glucose-Flux VSOJ (2008) 2-IV-23 Sonoda-11

Conclusion • Nutritional oxidative stress, or postprandial oxidative stress ensues from an imbalance between the reactive oxygen species generated and the antioxidative defence system. • Endogenous antioxidative capacity influenced by glucose-flux can be recovered immediately in young, but remains a low-level in elder without adequate supplementation of antioxidative vitamins. • The method employing a photosensitization technique would estimate comprehensively the endogenous antioxidative capacity, or the redox balance. VSOJ (2008) 2-IV-23 Sonoda-12

指頭血による生体酸化包括的評価法と 糖質摂取惹起酸化ストレスの ビタミン C による抑制 塩入 麻由 1 , 井上 葉子 1,2 , 矢部 えん 1