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Microtechnologies for biodetection and diagnostics

Peter Lillehoj, Ph.D. Microtechnologies for biodetection and diagnostics. November 27, 2012. Introduction to MEMS. Micro-electrical-mechanical systems Started in mid-late 1980’s Typical component size: 1-100s µm. http://biopoets.berkeley.edu. www.eee.metu.edu. http://joebrownmems.com.

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Microtechnologies for biodetection and diagnostics

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  1. Peter Lillehoj, Ph.D. Microtechnologies for biodetection and diagnostics November 27, 2012

  2. Introduction to MEMS • Micro-electrical-mechanical systems • Started in mid-late 1980’s • Typical component size: 1-100s µm http://biopoets.berkeley.edu www.eee.metu.edu http://joebrownmems.com

  3. BioMEMS • Manipulate, analyze or process biological samples • Improved performance over conventional technologies S. Sia Lab, Columbia University http://www.smartsheet.com L. Lee Lab, UC Berkeley http://www.dolomite-microfluidics.com

  4. Microfabrication Etching Patterning Molding PDMS Si wafer Deposition

  5. Microfluidic systems: cell trapping & lysis Buffer flow rate 10 µL/min 0 µL/min 15 µL/min Trapping efficiency % Buffer flow rate (µL/min) C. Wu, P. B. Lillehoj, et al., Biotechnology Journal, 2011

  6. Microfluidic systems: cell sorting Separation region C1 R1 P1 P2 P3 Sample inlet C2 R2 S1 S2 Sheath flow inlet C3 R3 P. B. Lillehoj, et al., Lab on a Chip, 2010

  7. Biosensing I: capturing Contact pads Targeting domain Peptides Sensors C Binding domain Alignment mark Au sensor

  8. Biosensing II: detecting P. aerugionsa S. mutans E1 E2 E3 E4 E1 E2 E3 E4 P. B. Lillehoj, et al., Proc. Of 6th IEEE NANOMED 2012

  9. Lab-on-a-chip • Fluids are autonomously driven within channels via capillary pumping • Detection is carried out using a highly-sensitive rapid electrochemical sensor Capillary Pump Inlet 1 Outlet Mixer Inlet 2 Sensor P. B.Lillehoj, et al.,Lab on a Chip, 2010

  10. Electrochemical detection: w/ target current time TMB TMB

  11. Electrochemical detection: w/o target current time TMB TMB

  12. BoNT detection results BoNT/A Specificity BoNT/A Detection Limit • BoNT/A could be detected at concentrations down to 5 ng/mL with high specificity • The entire detection process only requires 2 loading stepsand can be completed within 20 min

  13. Cell phone diagnostics • Highly portable • Cell phones contain a visual display, electrical interface and power source • Widely accessible • >75% of the world are mobile subscribers3 www.mobiledia.com www.wired.com www.dipity.com/tickr/ 3http://www.grandchallenges.org/

  14. Summary & Conclusions • Microtechnologies enables for the development of next-generation systems

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