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Senior Design Group 12

Senior Design Group 12. Team Members: Jon Sunderland, Justin Mai, Kyle Petersen, Prateek Bhatnagar , Umair Ilyas Project Advisor: Santosh Pandey Co-Advisor: Umesh Vadya Graduate Student Advisors: Archana Parashar , John Carr.

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Senior Design Group 12

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  1. Senior Design Group 12 Team Members: Jon Sunderland, Justin Mai, Kyle Petersen, PrateekBhatnagar, UmairIlyas Project Advisor: SantoshPandey Co-Advisor: UmeshVadya Graduate Student Advisors: ArchanaParashar, John Carr Identifying Behavioral Patterns of Living Microorganisms Using Microfluidic Systems

  2. Outline • Project Plan • Goals • Problems • Solutions • Design • System Design • Detailed Design • Implementation • Prototype • Project Closure • Project Success • Questions & Answers

  3. Why? • Parasitic nematodes are harmful for the environment • Understanding the behavior of nematodes in order to prevent their negative effects Why C. elegans? • C. elegans is a transparent nematode whose genome has been mapped completely http://www.divergence.com/ma_crop_protection.php

  4. Problems • How to get useful work out of microorganisms • Finding response of C. elegans to different taxes • Finding effective ways of mixing immiscible and miscible chemicals at a micro level • Producing results from data coming out of the tracking software

  5. Solutions/Project Goals • Identify, characterize, and understand behavioral patterns of microorganisms by designing and fabricating microfluidic chips • Design liquid diffusion biomotors • Create data analysis GUI

  6. Concept Sketch • Concept: Use taxes to develop biomotors • Electrotaxis: response to electric fields • Magnetotaxis: response to magnetic fields • Geotaxis: response to gravitational fields

  7. Functional Requirements • Determine and quantify the effects of magnetic fields (magnetotaxis) on C. elegans • Design and test useful biomotor applications • Produce work • C. elegans sort themselves • Develop procedures to perform these experiments

  8. Functional Requirements • Create an apparatus that is necessary but unavailable • Holders with specific dimensions, magnetic properties or optical properties • Design microfluidic chip layout • Obtain accurate experimental data • Ethical research, which includes quality of research, ethical conduct, proper acknowledgements

  9. Non-Functional Requirements

  10. Non-Functional Requirements • Intuitive data analysis • Easy-to-use universal design • Robust for adding additional plots • Significantly reduced plotting times

  11. Fabrication of Microfluidic Chips Soft Lithography Process: • Master Mold Fabrication • Polymer Molding

  12. Market & Literature • Previous taxes studied include electrotaxis, phototaxis, chemotaxis, phonotaxis, and some geotaxis • Magnetotaxis has not been fully understood

  13. Market & Literature • C. elegans genotypes well studied, phenotypes not so much • C. elegans cheap, easy to store, simple, and transparent • Knowledge of previous research is important • Our research needs to be new and useful Figure: C.elegans in microfluidic channel

  14. Resource & Cost Estimate

  15. Project Schedule (Gantt Chart)

  16. Potential Risks and Mitigation Strategies All risks that can be foreseen can be avoided through proper procedure in the lab. • There are few chemicals that are dangerous and if those are properly stored then no one is in danger of exposure. • Proper procedure was determined so that all participants were aware of the risks. • One can seriously injure oneself while working with extremely strong magnets. Magnets should be handled properly

  17. System Design

  18. Functional Decomposition • Project flow highlights biomotor design • Using multiple taxes

  19. Technology Platforms • Software • Microscope imaging software – Q-Imaging & Q-Capture Pro • Worm tracking – Worm Tracker V2.1.3 & Analyzer • Matlab • Hardware • Microscopes integrated with cameras • Strong permanent magnets of varying strengths (magnetotaxis) • Custom designed magnet holders • Misc. equipment include glass slides, forceps, masks, chip designs etc.

  20. Magnetotaxis Experiment Design Stage 1: Chip Design • Main goal, to eliminate bias in C. elegans’ behavior

  21. Magnetotaxis Experiment Design • Understand relation of field and behavior • 6 data points Stage 2: Determine Effect of Field Strength

  22. Magnetotaxis Experiment Design • Prefer North? Prefer North to South? Stage 3: Determine Effect of Field Polarity

  23. Magnetotaxis Experiment Design Stage 4: Determine Amount of Control • Experiment: Reverse polarity, observe response rate Jon Please add two most relevent result graphs on magnetotaxis

  24. Magnetotaxis Experiment Design Stage 4: Determine Amount of Control • Experiment: Reverse polarity, observe response rate Jon Please add two most relevent result graphs on magnetotaxis

  25. What are Biomotors • Molecular Motors/Molecular walking machines

  26. Biomotor Design • Stage 1: Developing Ideas for biomotor uses • Key objective is to find how C. elegans can be used as biomotors • Integrate the taxis results into biomotor design

  27. Biomotor Design • Stage 2: Designing Microfluidic Chips for the Specific Application • Stage 3: Testing Chip Design • Stage 4: Optimization of the Biomotor Figure : Fluid Flow/Diffusion Biomotor Design

  28. Implementation • Two prototype biomotor designs tested for mixing of drugs • First design with straight channel was successful

  29. Design Tradeoffs • Design modification had to be done to the second design • Increasing the width of the main channel • Decreasing the side angles to 30 ° as opposed to 45°

  30. Software Implementation

  31. Data Analysis & Results

  32. Data Analysis & Results

  33. Project Success and Future Work • Team was able to demonstrate a workable biomotor design which was the end goal of the project • Additionally the team came up with eight biomotor designs which can be tested by future teams working on same or similar projects

  34. Questions & Answers

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