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SOD Research 5/09

SOD Research 5/09. - SOD Spore Surveys & SOD Blitz / Outreach - Phosphonate Treatment of Tanoaks and CLOs - Long Term Efficacy of SOD Treatments - Resistance / Tolerance in Tanoaks Transmission of SOD Through Pruning Management Planning. SOD Spore Monitoring.

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SOD Research 5/09

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  1. SOD Research 5/09 • - SOD Spore Surveys & SOD Blitz / Outreach - Phosphonate Treatment of Tanoaks and CLOs - Long Term Efficacy of SOD Treatments - Resistance / Tolerance in Tanoaks • Transmission of SOD Through Pruning • Management Planning

  2. SOD Spore Monitoring 8 Field Monitoring Sites 16 Buckets / Site 5 Leaves / Bucket = 640 leaves sampled every 3 weeks Starting again in February 08

  3. Bay Laurel / Tanoak SOD Spore Survey Temp (C) Rain (mm) Date

  4. SOD Blitz – Disease Monitoring

  5. SOD Blitz / SOD Treatment Training Sessions

  6. Injection Treatment Topical Treatment

  7. Save the Tanoaks Project SOD Treatment Sites

  8. Long Term Efficacy of SOD Treatments

  9. Long Term Efficacy of SOD Treatments Agrifos vs. Azomite Treatments Azomite & Limewash Canker Size (% of Untreated Positive Control) Agrifos & PentraBark Novato Mature CLOs Detached Branch Assay

  10. Effect of Tree Size on Treatment Efficacy

  11. Tanoak Common Garden Experiment Shadehouse

  12. Tanoak Out Planting – Common Garden Experiment

  13. Tanoak Assays Whole Seedling Inoculation Tanoak Petiole Plug Assay Untreated Control Tree Infected Seedling Phosphonate Treated Tree

  14. Whole-seedling inoculation 9 8 7 6 Mean Lesion Length, Apr09 5 4 3 2 1 0 1 2 3 4 5 6 8 9 11 13 14 15 16 17 18 19 21 23 24 25 50 Family

  15. Zoospore Inoculator for P. Ramorum 1 cm Zoospores are in Direct Contact with Tree for 24 hrs. Grafting Wax Creates a Water Tight Seal

  16. Transmission of SOD through pruning: ► Does tree pruning introduce SOD infection? ► Is the timing of cutting a factor? Test at: 1 week 1 month 2 months 4 months Pruning CLO Branches Zoospore Inoculator Grady Ranch Site

  17. Transmission of SOD Through Pruning

  18. Transmission of SOD Through Pruning Grady Ranch 11/27/07

  19. Air Sampling for SOD Spores

  20. Steam Baiting for SOD Monitoring

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