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Direct Electronic Identification of Oligonucleotides with Inelastic Electron Tunneling Spectroscopy

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Direct Electronic Identification of Oligonucleotides with Inelastic Electron Tunneling Spectroscopy

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    1. Direct Electronic Identification of Oligonucleotides with Inelastic Electron Tunneling Spectroscopy John Lund, Declan Ryan, Ranjana Mehta, Maryam Rahimi and Babak A. Parviz Center of Excellence in Genomic Sciences Microscale Life Sciences Center University of Washington USA

    3. What do we need to detect?

    4. All-Electronic Sequencing

    5. How Fast Can STMs Work?

    6. Inelastic Tunneling Spectroscopy

    7. Inelastic Tunneling Spectroscopy

    8. Inelastic Tunneling Spectroscopy

    9. Molecular Extension

    10. Experimental Details

    11. Procedure

    12. Procedure

    13. Procedure

    14. Procedure

    15. 3D AFM image of bare HOPG before combing DNA Results

    16. 3D AFM image of ? phage ds-DNA completely elongated on HOPG with molecular combing. The DNA goes over multiple domains on the graphite surface.

    17. 3D AFM image of coiled ? phage ss-DNA deposited on HOPG prior to molecular combing.

    18. 3D AFM image of ? phage ss-DNA completely elongated on HOPG after the completion of the molecular combing procedure.

    21. Tunneling spectroscopy on gold

    22. Spectroscopy on poly A’s

    23. Spectroscopy on poly C’s

    24. Spectroscopy on poly G’s

    25. Spectroscopy on poly T’s

    26. Deviation from blank gold

    27. Confirmation of IETS

    28. Measurement on stretched dsDNA

    29. Tip steering approach

    30. Conclusions All-electronic genome sequencing requires cost-effective and reproducible methods for extension of DNA on atomically flat surfaces Molecular combing offers a simple and cost-effective method for stretching DNA on surfaces IETS is a promising method for identifying DNA bases on conductive substrates using STM We have measured IETS spectra on 5-mer DNA bases on gold and will apply our approach to sequencing strands of DNA in the future

    31. Acknowledgments

    32. Undigested ? phage ds-DNA on HOPG

    33. ds-DNA Hind III digest on HOPG with 10 mM MgCl2

    34. ? phage ss-DNA Hind III digest on HOPG with 10 mM MgCl2

    35. STM imaging of ssDNA on HOPG

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