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Größenbeobachtung A498 Sphäroide- unbehandelt

Größenbeobachtung A498 Sphäroide- unbehandelt. 1. RAD001 Behandlung. IF 3 EJ28 – Formalinfixierung 1° AK: 1:50 2° AK: 1:30. neg. control. FLT1. PDGFR- α. IF 3 A498 – Formalinfixierung 1° AK: 1:50 2° AK: 1:30. neg.control. FLT1. PDGFR- α. FLT-1 neg. contr. IF4

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Größenbeobachtung A498 Sphäroide- unbehandelt

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  1. Größenbeobachtung A498 Sphäroide- unbehandelt

  2. 1. RAD001 Behandlung

  3. IF 3 EJ28 – Formalinfixierung 1° AK: 1:50 2° AK: 1:30 neg. control FLT1 PDGFR-α

  4. IF 3 A498 – Formalinfixierung 1° AK: 1:50 2° AK: 1:30 neg.control FLT1 PDGFR-α

  5. FLT-1 neg. contr. IF4 FLT-1 Konzentrationsoptimierung FLT-1 1:25 FLT-1 1:50 FLT-1 1:33

  6. PDGFR-α neg. contr. IF5 PDGFR-αKonzentrationsoptimierung PDGFR-α 1:25 PDGFR-α 1zu50 PDGFR-α 1zu33

  7. PDGFR-β neg. contr. IF5 PDGFR-βKonzentrationsoptimierung PDGFR-β 1:25 PDGFR-β 1:50 PDGFR-β 1:33

  8. PDGFR-β 1:25 FITC und DAPI kombiniert

  9. Optimierung Western Blot p70S6K 70-85 kDa, rabbit P-p70S6K 70-85 kDa, rabbit; P-Thr389 4E-BP1 15-20 kDa, rabbit P-4E-BP1 15-20 kDa, rabbit; P-Thr70

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