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Particle Size Sizing Technique 2: hydrodynamic chromatography sieving microscopy

Particle Size Sizing Technique 2: hydrodynamic chromatography sieving microscopy. Kausar Ahmad Kulliyyah of Pharmacy, IIUM http://staff.iiu.edu.my/akausar. Hydrodynamic Chromatography. HDC Operation. Results generated by HDC. Liposome PL-PSDA (Polymer Lab: HDC mechanism).

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Particle Size Sizing Technique 2: hydrodynamic chromatography sieving microscopy

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  1. Particle Size Sizing Technique 2: hydrodynamic chromatography sieving microscopy Kausar Ahmad Kulliyyah of Pharmacy, IIUM http://staff.iiu.edu.my/akausar Physical Pharmacy 2

  2. Hydrodynamic Chromatography Physical Pharmacy 2

  3. HDC Operation Physical Pharmacy 2

  4. Results generated by HDC Physical Pharmacy 2

  5. LiposomePL-PSDA (Polymer Lab: HDC mechanism) What is the volume average diameter? What is the polydispersity index? Physical Pharmacy 2

  6. Sieving • POWDER can be separatedinto various size fractions by vibrating sieve loaded with sample to enable the particles of size less than that of the mesh openings to pass throughand the over size to remain in the sieve. • Dry sieving is adopted for free flowing powder samples of size range varying from e.g. 4mm down to 25μm. Physical Pharmacy 2

  7. Sieves • A standard test sieve is generally made of a woven wire mesh cloth • specified wire thickness with square openings • Standard sieves are made according to recommended norms to maintain opening size interval between successive sieves. • The ratio between successive sieves is kept as a constant such as 1.414 and hence the sieve size varies in geometric progression. • fixed to a rectangular or circular frame Physical Pharmacy 2

  8. Sieving procedure Sieves stacked Maximum size g f e Wire mesh d c b a pan Minimum size Physical Pharmacy 2

  9. Table of Sieve Results Physical Pharmacy 2

  10. Interpretation of Sieve Results • From the % cumulative weight, the mean diameter can be obtained i.e. • d(0.5) – the diameter at which 50% of the samples, based on weight falls below it. Cumulative weight (%) Weight (%) Physical Pharmacy 2

  11. Microscopyhttp://www.mos.org/sln/SEM/index.html The deer tick, is about the size of a freckle. To fly, moths must be light. A close-up of the Cecropia Moth scale shows that it is mostly air, adding very little weight to the moth The shells of radiolarians, single-celled animals. Physical Pharmacy 2

  12. Principles of Microscopyhttp://micro.magnet.fsu.edu/primer/lightandcolor/polarizedlighthome.html Physical Pharmacy 2

  13. Using light microscopy equipped with graticule to determine size Physical Pharmacy 2

  14. Comparison of microscopes Physical Pharmacy 2

  15. Scanning Electron Microscope Physical Pharmacy 2

  16. SEM Technique Physical Pharmacy 2

  17. Inside the SEM Physical Pharmacy 2

  18. Information given by SEM Physical Pharmacy 2

  19. References SL Flegler, JW Heckman, KL Klomparens, Scanning and transmission electron microscopy, Oxford, New York (1993) http://micro.magnet.fsu.edu/primer/lightandcolor/polarizedlighthome.html Physical Pharmacy 2

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