Microfluidic 3D Bioprinter: Problem Definition

1. Microfluidic 3D Bioprinter: Problem Definition Nicole Mazzola, Matt Williams, Matt Freyman, Aaron Gaylord, Reed Truax, Taylor Fowler, Moises Gomez Aka “The Idipsumographers”

2. Agenda • Team Introduction, Values and Norms • Background and Summary • Problem Statement • Project Key Goals and Deliverables • Use Scenarios • Stakeholders • Customer & Engineering Requirements • Constraints • Benchmarking • Plans for next phase • Project Risks • Questions & Acknowledgements

3. Team: The Idipsumographers

4. Values and Norms Attendance: Team members are expected to: • Show up promptly to all planned meetings. • Notify team members in advance if you will miss a meeting. • Team will not try to schedule excessive meetings and only include necessary members. Honesty/Ethics: All team members are expected to: • Follow RIT’s ethics policy. • Expected to be honest and ethical in their behaviour and decision making. Accountability / Responsibility: Individual team members are responsible for: • Assigned work meeting set deadline. • Responsible to notify all team members If issues arise. Enthusiasm: Team members are expected to: • Encourage team dynamics, enthusiasm, and have a positive attitude towards the project.

5. Values and Norms Communication: Team members are expected to: • Notify the group beforehand, • Emails should be responded to within 24 hours. • Texts/calls & Slack within 12 hours. • Keep team aware of their schedule and progress on a weekly basis. Decisions: • All decisions will be made by majority rule. • If a tie arises the team leader will break that tie. • If the issue is significant, then the customer may be included. Documentation: Team members will document any and all ideas, and work will include: • Proper references and citation where applicable. • All documentation will be shared on the team’s google drive. • Final documentation will be uploaded to EDGE.

6. 3D Bioprinter Summary & Background 3D printing, otherwise known as additive manufacturing was: • First developed in the mid 1980's and has been • Transforming what's possible in the manufacturing world. • Redefining how parts are designed. Additive manufacturing technology has been. • Adopted by the biomedical research field as a possible way of "printing" organs and other biological matter. Current bio printers for lab work: • Cost upwards of $100,000. • Have difficulty printing multiple materials at a time. Project is focused on: • Retrofitting an off-the-shelf printer to print biomaterials. • Utilize a microfluidic process called hydrodynamic focusing. • Develop disposable printheads allowing a printer to cheaply and easily print multiple biomaterials out of a single printhead. This design and prototype will serve as a proof of concept for future iterations which will be able to print multiple materials, including cells, in three dimensions, in a sterile environment.

7. Problem Statement Desired State • Cost effective • Desktop printer for academic use • Ability to print multiple materials at once • Flexibility in reservoir volume • Extrude crosslinked gel Current State: • $10,000-$200,000 • Large scale • Not many materials on once • Few desktop models • Post- crosslinking • Limited reservoir volume

8. Project Goals and Key Deliverables Goals: • Create a working prototype • Consistent prints • Easy to use Interface • Print right from slicing software • Inexpensive • Portable • Short print time Deliverables: • Detailed design plans • Video demonstrations • Preliminary print head design • Solution Documentation • Multiple printheads and documentation • Scientific publication • User guide for setup and operation

9. Stakeholders

10. Benchmarking

11. Customer & Engineering Requirements

12. Use Scenarios

13. Plan for MSD I

14. Plan for Next Phase

15. Project Risks • Mechanical: Concerns about material path and printing head. • Resource: Scheduling meetings or time in labs. • Resource: How much room for development is there in our budget? • Safety: Proper insulation, ventilation, and personal protective equipment (PPE). • Environmental: Proper recycling of unneeded printer parts/used printer parts (i.e. acrylic printer heads). • Environmental: Design of disposal system for excess printing material.

16. Questions? • ?

17. Acknowledgements Vinay Abhyankar - Our Customer BME department - Support and funding Chris Leibfried - Our Guide

18. Picture Citations • https://www.google.com/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=2ahUKEwiSn7nl1bPdAhWQVt8KHZpSAkcQjRx6BAgBEAU&url=https%3A%2F%2Fcellink.com%2Fproduct%2Fcellink-bio-x-bioprinter%2F&psig=AOvVaw3kTWn23a81jFrJuB3ih5gN&ust=1536779983952059 • https://3dprintingindustry.com/wp-content/uploads/2015/08/bioplotter-manufacturer-e1423774692322.jpg • https://www.elveflow.com/wp-content/uploads/2015/01/recirculating-perfusion-microfluidic-system.jpg