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This project delves into the phenomenon of osmosis in biological systems, specifically focusing on plasmolysis in plant cells. Learn the key definitions and differences between osmosis and diffusion as well as the concepts of isotonic, hypotonic, and hypertonic solutions. Follow a detailed experiment protocol to observe plasmolysis in Rhoeo discolor leaf cells, estimating the isotonic concentration through sucrose dilutions. Engage with interactive microscopy to study cellular changes and enhance your understanding of osmosis at the cellular level.
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Observing Osmosis in Biological Systems Plasmolysis
Some definitions Osmosis: Passage of water from a region of low concentration of solute to a region of high concentration, across a semipermeable membrane How is Osmosis different from Diffusion?
Isotonic: Same solute concentration Hypotonic: Low solute concentration Hypertonic: High solute concentration
Protocol • Prepare sucrose dilutions from the 1M stock provided. • Peel off a thin layer of the leaf from the purple side ( purple layer only) • Put into the sucrose solution and wait for 20 minutes. • Place the layer on the microscope slide, place a coverslip and observe the cells under microscope. • Determine the number of plasmolysed cells in different concentrations of sucrose and estimate the isotonic concentration.