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Observing Osmosis in Biological Systems Plasmolysis

Observing Osmosis in Biological Systems Plasmolysis. Some definitions. Osmosis: Passage of water from a region of low concentration of solute to a region of high concentration, across a semipermeable membrane How is Osmosis different from Diffusion?. Isotonic: Same solute concentration

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Observing Osmosis in Biological Systems Plasmolysis

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  1. Observing Osmosis in Biological Systems Plasmolysis

  2. Some definitions Osmosis: Passage of water from a region of low concentration of solute to a region of high concentration, across a semipermeable membrane How is Osmosis different from Diffusion?

  3. Isotonic: Same solute concentration Hypotonic: Low solute concentration Hypertonic: High solute concentration

  4. Osmosis in plant cells

  5. Osmosis in blood cells

  6. Osmosis in plant cells - Plasmolysis

  7. ExperimentTo observe plasmolysis in Rhoeo discolor

  8. Protocol • Prepare sucrose dilutions from the 1M stock provided. • Peel off a thin layer of the leaf from the purple side ( purple layer only) • Put into the sucrose solution and wait for 20 minutes. • Place the layer on the microscope slide, place a coverslip and observe the cells under microscope. • Determine the number of plasmolysed cells in different concentrations of sucrose and estimate the isotonic concentration.

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