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The Effect of Liquefaction on the Ability of Semen and Semen Amyloid Fibrils to Enhance HIV Infection. Nadia Roan, PhD International AIDS Meeting 2012 July 23, 2012. Title Box. Title Box. Title Box. Title Box. Title Box. Title Box.
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The Effect of Liquefaction on the Ability of Semen and Semen Amyloid Fibrils to Enhance HIV Infection Nadia Roan, PhD International AIDS Meeting 2012 July 23, 2012
During semen liquefaction, PSA specifically cleaves SEM proteins
Title Box +AEBSF Mock Hours incubation: 0
Summary • Fragments from semenogelins form amyloid fibrils and enhance HIV infection
Summary • Fragments from semenogelins form amyloid fibrils and enhance HIV infection • Semen samples naturally defective in semenogelins do not enhance HIV infection
Summary • Fragments from semenogelins form amyloid fibrils and enhance HIV infection • Semen samples naturally defective in semenogelins do not enhance HIV infection • Semen that has undergone prolonged liquefaction has diminished ability to enhance HIV infection. The “window of opportunity” for viral enhancement activity is >8 hours post-emission
Acknowledgements Gladstone Institute of Virology and Immunology Warner Greene Simon Chu Greene Lab Members University of California, San Francisco Ewa Witkowska Haichuan Liu University of Ulm Frank Kirchhoff Jan Münch Janis Müller A.P. Giannini Medical Research Foundation 1PO1 AI083050-01 PPG Department of Defense #10113005