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SmaI XmaI SalI BbvII DraIII EcoO109 1 PpuMI BamHI BspM II PflM I. TnPhoA. Tn5Int. IS50. phoA. MCS. IS50R. IS50L. neomycin. Tn5Int (TnPhoA Sequencing Primer): 5’-CGGGAAAGGTTCCGTTCAGGACGC-3’.
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SmaI XmaI SalI BbvII DraIII EcoO109 1 PpuMI BamHI BspM II PflM I TnPhoA Tn5Int IS50 phoA MCS IS50R IS50L neomycin Tn5Int (TnPhoA Sequencing Primer): 5’-CGGGAAAGGTTCCGTTCAGGACGC-3’ See Manoil, C. and Beckwith, J. (1985) TnphoA: A transposon probe for protein export signals. Proc. Natl. Acad. Sci USA82: 8129-33.