ISS INCREMENT 27/28 Human Life Science USOS Payload Complement March 2010

1. ISS INCREMENT 27/28 Human Life Science USOS Payload ComplementMarch 2010 Committee for the Protection of Human Subjects (CPHS) Japan Aerospace Exploration Agency Institutional Review Board(JAXAIRB) Human Research Multilateral Review Board (HRMRB) European Space Agency Medical Board (ESA MB) Canadian Space Agency (CSA)

2. ISS INCREMENT 27/28 OVERVIEW Inc 28 Inc 27 Inc 26/27 (Kondratiev – FE) Inc 28/29 (Fossum – FE4) Inc 28/29 (Furukawa – FE5) Inc 26/27 (Coleman – FE) Inc 28/29 (Volkov – FE6) Inc 26/27 (Nespoli – FE6) Inc 27/28 (Samokutyayev – FE1/CDR) Inc 28/29 Inc 25/26 Inc 27/28 (Garan – FE2) Inc 27/28 (Borisienko -- FE3) 42P Apr 10 26S Mar 30 43P Apr 30 27S May 30 44P Jun 30 45P Aug 25 COTS Mar 27 Orbital D1 HTV3 Jul 1 CRS Aug 1 SpaceX-2 CRS May 1 SpaceX-1 24S Rtn Mar 16 25S Rtn May 15 26S Rtn Sep 14

3. INCREMENT 27/28 PROTOCOLS ISSMP Pre/In/Post-flight Experiments • E255/SMO-021/Bisphosphonates as a Countermeasure to Spaceflight Induced Bone Loss (Bisphosphonates) • Adrian LeBlanc, Ph.D.; USRA, Houston • Toshio Matsumoto, M.D., Ph.D.; University of Tokushima, Japan • E377/Cardiac Atrophy and Diastolic Dysfunction During and After Long Duration Spaceflight: Functional Consequences for Orthostatic Intolerance, Exercise Capacity, and Risk of Cardiac Arrhythmias (Integrated Cardiovascular) • Benjamin D. Levine, M.D.; Institute for Exercise & Environmental Medicine, Dallas, TX • Michael W. Bungo, M.D.; UT Medical School, Houston, TX • SMO-015/Validation of Procedures for Monitoring Crewmember Immune Function (Integrated Immune) • Clarence Sams, Ph.D.; NASA-JSC • Integrated Resistance and Aerobic Training Study (iRATS) • Lori Ploutz-Snyder, Ph.D.; USRA, Houston • Nutritional Status Assessment (Nutrition) • Scott Smith, Ph.D.; NASA-JSC

4. INCREMENT 27/28 PROTOCOLS, cont. ISSMP Pre/In/Post-flight Experiments (cont.) • Dietary Intake Can Predict and Protect Against Changes in Bone Metabolism During Spaceflight and Recovery (Pro K) • Scott Smith, Ph.D.; NASA-JSC • Psychomotor Vigilance Self Test on ISS (Reaction Self Test) • David F. Dinges, Ph.D.; University of Pennsylvania • ISSMP/NASA Biological Specimen Repository (Repository) • Curator: Kathleen McMonigal, M.D.; NASA-JSC • Evaluation of Maximal Oxygen Uptake (VO2max) and Submaximal Estimates of VO2max Before, During and After Long Duration International Space Station Missions (VO2max) • Alan D. Moore, Jr., Ph.D.; Wyle Laboratories, Houston ISSMP Pre/Post-flight Experiment • Physiological Factors Contributing to Postflight Changes in Functional Performance (Functional Task Test) • Jacob J. Bloomberg, Ph.D.; NASA-JSC

5. INCREMENT 27/28 PROTOCOLS, cont. CSA Pre/In/Post-flight Experiments • Cardiovascular Health Consequences of Long-Duration Space Flight (Vascular) • Richard Hughson, Ph.D.; University of Waterloo, Ontario, Canada • ESA Pre/In/Post-flight Experiments • Mental Representation of Spatial Cues during Spaceflight (3D Space) • Gilles Clément, Ph.D; CNRS-CerCo Faculte de Medecine Rangueil, Toulouse, France • Assessment of Endurance Capacity by Gas Exchange and Heart Rate Kinetics during Physical Training (EKE) • Uwe Hoffmann, Ph.D.; German Sport University, Cologne, Germany • Astronaut’s Energy Requirements for Long-term Space Flights (Energy) • Stéphane Blanc, Ph.D.; Centre d’Ecologie et Physiologie Energétiques

6. INCREMENT 27/28 PROTOCOLS, cont. ESA Pre/In/Post-flight Experiments • Scaling Body-Related Actions in the Absence of Gravity (Passages) • Marion Luyat, Ph.D.; Centre National de la Recherche Scientifique (CNRS), France • Sodium Retention in Microgravity (SOLO) • Martina Heer, Ph.D.; DLR - Institute of Aerospace Medicine, Cologne, Germany • Core Temperature Changes in Humans Before, During, and After Exercise Performed on the International Space Station (Thermolab) • Hanns-Christian Gunga, Ph.D.; Center for Space Medicine Berlin Campus Benjamin Franklin Charité Universitätsmedizin • Vascular Echography (Vessel Imaging) • P. Arbeille, Ph.D.; University Hospital Trousseau Tours, France ESA Pre/Post-flight Only Experiments • Early Detection of Osteoporosis in Space (EDOS) • Laurence Vico, M.D.; Faculté de Médecine • Validation of Centrifugation as a Countermeasure for Otolith Deconditioning during Spaceflight (SPIN) • Floris Wuyts, Ph.D.; University of Antwerp, Belgium

7. INCREMENT 27/28 PROTOCOLS, cont. JAXA Pre/In/Post-flight Experiments • Biomedical Analyses of Human Hair Exposed a Long-term Space Flight (Hair) • Chiaki Mukai, M.D., Ph. D.; JAXA - Tsukuba Space Center (TKSC) • Mycological Evaluation of Crew Exposure to ISS Ambient Air (Myco) • Chiaki Mukai, M.D., Ph. D.; JAXA - Tsukuba Space Center (TKSC) • Onboard Diagnostic Kit • TBD

8. TARGET MATRIX FOR INCREMENT 27/28 PROTOCOLS

9. INSTITUTIONAL REVIEW BOARD STATUS *=action items still open

10. PREFLIGHT TRAINING REQUIREMENTS Note: Times reflected in chart represent one crewmember being trained to proficiency.

11. PREFLIGHT TRAINING REQUIREMENTS, cont. Note: Times reflected in chart represent one crewmember being trained to proficiency.

12. PREFLIGHT TRAINING REQUIREMENTS, cont. Note: Times reflected in chart represent one crewmember being trained to proficiency.

13. IN-FLIGHT SCHEDULE Note: Times reflected in chart are for one subject.

14. IN-FLIGHT SCHEDULE, cont. Note: Times reflected in chart are for one subject.

15. IN-FLIGHT SCHEDULE, cont. Note: Times reflected in chart are for one subject.

16. IN-FLIGHT SCHEDULE, cont. Note: Times reflected in chart are for one subject.

17. INCREMENT 27/28 COMPLEMENT ASSESSMENT • It will not be possible for crewmembers to perform all investigations for which they have been targeted. • Reasons include: • Postflight BDC scheduling conflicts • Science conflicts between experiments • The intention remains to maximize science opportunities for all agencies and the same approach as I25/26 utilizing the crew interest survey will be implemented. • The prime crewmembers for 27/28 are backups to the 25/26 crewmembers and have been briefed on most of the experiments being offered. • The interest survey will show all experiments requesting the crewmember’s participation, and though they can indicate interest in different experiments from what they are participating in as backups, it is reasonable to assume that they will remain in nearly the same complements.

18. CREW INFORMED CONSENT BRIEFING • Crewmembers will be briefed on all experiments targeted for their participation (if previously briefed, crewmembers will not receive the presentation again unless they wish to or significant changes have been made). • Survey responses will be requested within one week. If a response has not been received by that time, the Increment Science Coordinator (ISC) will contact the crewmember to answer any questions he/she may have. • Once all surveys are received, ISSMP, International Partners and the NASA Payloads Office will develop the best-fit scenario for each crewmember. • Each crewmember will then be shown his/her proposed complement and consent form signatures will be requested. • The CPHS will be provided a participation matrix once the process is complete.

19. COMPLEMENT SCENARIOS Note: Bisphosphonates subjects cannot participate in Pro K, SOLO or EDOS

20. BLOOD VOLUMES • The blood volumes of all experiments combined does not exceed the limit during any of the pre- or postflight timeframes. • For all scenarios, the scheduling of inflight operations for all experiments will be closely monitored so as to not break the 100 mL per 30 days rule.

21. COMPLEMENT SCENARIOS 1 & 2 BLOOD VOLUMES

22. COMPLEMENT SCENARIOS 3 & 4 BLOOD VOLUMES NOTE: The difference in blood volume in these scenarios are the exclusion of Vascular

23. COMPLEMENT SCENARIOS 5 BLOOD VOLUMES NOTE: The difference in blood volume in these scenarios are the exclusion of FTT and inclusion of EDOS

24. COMPLEMENT SCENARIOS 6 & 7 BLOOD VOLUMES NOTE: The difference in blood volume in these scenarios are the exclusion of FTT and Vascular

25. BLOOD VOLUME BY ACTIVITY

26. BLOOD VOLUME BY ACTIVITY, cont.

27. BLOOD VOLUME BY ACTIVITY, cont.

28. BLOOD VOLUME BY ACTIVITY, cont.

29. BLOOD VOLUME BY ACTIVITY, cont.

30. Preflight and Postflight Baseline Data Collection Schedules