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Old Title vs New Title

Old Title vs New Title. NIH Guidelines for Research Involving Recombinant DNA Molecules. NIH Guidelines for Research Involving Recombinant or Synthetic Nucleic Acid Molecules Implement by 3/3/13. Is chemical synthesis covered?. synthesizer Not until in a ….

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Old Title vs New Title

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  1. Old Title vs New Title • NIH Guidelines for Research Involving Recombinant DNA Molecules • NIH Guidelines for Research Involving Recombinant or Synthetic Nucleic Acid Molecules • Implement by 3/3/13

  2. Is chemical synthesis covered? synthesizer Not until in a …

  3. III-F Registrations • Nothing exempt in Boston • BPHC adopts updated Guidelines unless… • Using nonhazardous plasmids (pBR) to clone gene of interest into host (nonpathogenic E.coli) • Often the first step in creating a transgenic animal • New exemptions for synthetic NA • No origin of replication, no integration…

  4. NIH Guidelines: Definition of rNA • Molecules constructed by joining nucleic acids • & can replicate in a living cell

  5. NIH Guidelines: synthetic • Nucleic acids that can base pr w naturally occurring nucleic acids

  6. NIH Definitions Continued • Molecules resulting from replication of rNA or synthetic nucleic acids in past two slides

  7. Other Changes • Fewer AAV serotypes need review • Transfer of drug resistance traits • Human Gene Transfer w nucleic acids

  8. Risk Assessment & Synthetics • % of genome from each parent • Fn/purpose of each sequence • Assume same fn as original host? • Synergism between sequences & transgenes

  9. What Next? - XNA • ~6 sugars can form NA bbone • Store & retrieve genetic info • Medical benefit? Slower breakdown in stomach & bloodstream

  10. Real World Examples • iGEM 2006 • Jay Keasling

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