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CNR IAMC - Istituto per l’Ambiente Marino Costiero Messina, Italy Università degli Studi di Messina. Basin Description. State of the Art April 2003. 3380dbar. 3380dbar. DECREASING ENERGY YIELD. O 2 >. NO 3 >. MnO 2 >. (FeOH) 3 >. SO 4 2- >. CO 2. O 2 > Eh + 0.812 dG – 29.9.
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CNR IAMC - Istituto per l’Ambiente Marino Costiero Messina, Italy Università degli Studi di Messina Basin Description State of the Art April 2003
3380dbar DECREASING ENERGY YIELD O2> NO3> MnO2> (FeOH)3> SO42-> CO2
O2> Eh + 0.812 dG – 29.9 3380dbar Oxygen (CH2O)106(NH3)16(H3PO4) + 138O2 106CO2 + 16HNO3 + H3PO4 + 122H2O
NO3> Eh + 0.747 dG – 28.4 Nitrate 3380dbar (CH2O)106(NH3)16(H3PO4) + 94.4HNO3 106CO2 + 42.2 N2 + 16NH3
Manganese Barium MnO2> EH + 0.526 dG – 23.3 3380dbar MnO2 (solid) Oxic Ba++ Mn2+ (Dissolved) Anoxic Ba++ 236MnO2 + (CH2O)106(NH3)16(H3PO4) + 364 CO2 + 104 H2O 470 HCO3- + 8N2 + 236Mn2+ HPO42-
(FeOH)3> Eh –0.047 dG –10.1 3380dbar Fe2O3 + (CH2O)106(NH3)16(H3PO4) + 740 CO2 + 316 H2O 846 HCO3- + 424 Fe2+ 16NH3 + 8N2 + HPO42-
Sulphate Sulphide Sulphur SO42-> Eh - 0.221 dG – 5.9 3380dbar SO42- + (CH2O)106(NH3)16(H3PO4) 39 CO2 + 67HCO3- + 16NH4+ + 53HS- + 39H2O + HPO42-
CO2 EH – 0.224 dG – 5.6 3380dbar CO2 + H2O + CaCO3 Ca2+ +2HCO3-
Mg 3380dbar Cl K Na
3380dbar Jetten 2002 NH2OH + H2O NO + 5H+ + 4e-
Very different is the situation about the Discovery basin where the dissolution of the evaporite is just coming and where this young basin (about 2000 years ago) is filled of an homogeneous body brine with a very high concentration of magnesium chloride coming from the going bishofite dissolution (Wallmann, Nature 1997) The Discovery basin, where the dissolution of the evaporites is still going, and at date is envolving principally the bishofite, could be a start point of a just not living environment where the high magnesium chloride concentration avoid every kind of life locking the biotic degradation of organic compounds by microbial or esoenzimatic activity(ref). It is probably waiting for the complete dissolution of the evaporites to reach a liveble (vivibile?) environment.
CUBE MODEL • Deep • Salinity • Oxigen concentration • RedOx Potential • Methabolisms • Phylogenetic association
Species xxx Pmy 1 CFB Pmy 1
Planctomycetales 1 7A.LI B 64 2A.LI B 88 CLaquaticFormNullarborCavesAU UncUBA347051 CLAntarcContShelfSedAF424318 2A.LI B 82 UncPlanctomyceteAF424463 MarSnowAssCLagg27-L10943 UncBacAF328191 PIRULELLAsp PLANCTOMYCESsp UncPlanctomyceteAF424462 CLdeep-seaSedAB015544 UncCraterLakeAF316766 CLAntarcContShelfSedAF424490 UncPlanctomyceteAF513966 0.06 0.04 0.02 0.00
XXX SphingomonasMBIC3990 SPHINGOMONASyanoikuyaeX94099 1A.LI B 107 UncAlphaAF348719 ACIDOCELLAspX91797 CLSAR193 UncAlphaAY033309 HYPHOMONASjohnsonii SILICIBACTERlacuscaerulensisU7 strNKB7 CLDeepSeaSedToyamaBayAB077340 CLcoldMarineSedimentAJ240997 1A.LI B 14 0.04 0.02 0.00
Cytophagales CLsulfateRedConAF029039 IsolAustOilAJ011042 UncBacteroidBacteriumAY133094 6A.BB B 23 CLColdMarineSedAJ240979 CYTOPHAGAsp.UncAJ240979 1A.BB B 74 CytophagaAB015525 CYTOPHAGAsp.AB015525 CLsulfaRedConAF029041 CLAntarcMarLakeAF142822 CLAntMarLakeAF142822 CYTOPHAGAfermentansATCC19072 CYTOPHAGAfermentasATCC19072 CPHfermentans19072 CLHypersalineAlkalineLakeAF507 1A.BB B 1 CLAntarcMarLakeAF142901 CLAntMarLakeAF142901 1A.BB B 88 1A.BB B 31 3A.BB B 15 0.03 0.02 0.01 0.00
Plancotmycetales 2 1A.BB B 28 2A.BB B 47 UncCraterLakeAF316766 CLAntarcContShelfSedAF424490 UncPlanctomyceteAF513966 CLdeep-seaSedAB015544 UncPlanctomyceteAF424462 PIRULELLAsp 1A.BB B 107 PLANCTOMYCESsp UncBacAF328191 1A.BB B 34 1A.BB B 55 MarSnowAssCLagg27-L10943 UncPlanctomyceteAF424463 2A.BB B 56 2A.BB B 96 0.08 0.06 0.04 0.02 0.00
Species 7A.LI B 64 SphingomonasMBIC3990 2A.LI B 88 SPHINGOMONASyanoikuyaeX94099 CLaquaticFormNullarborCavesAU 1A.LI B 107 UncUBA347051 UncAlphaAF348719 CLAntarcContShelfSedAF424318 ACIDOCELLAspX91797 2A.LI B 82 CLSAR193 UncPlanctomyceteAF424463 UncAlphaAY033309 MarSnowAssCLagg27-L10943 HYPHOMONASjohnsonii UncBacAF328191 SILICIBACTERlacuscaerulensisU7 PIRULELLAsp strNKB7 PLANCTOMYCESsp CLDeepSeaSedToyamaBayAB077340 UncPlanctomyceteAF424462 CLdeep-seaSedAB015544 CLcoldMarineSedimentAJ240997 UncCraterLakeAF316766 1A.LI B 14 CLAntarcContShelfSedAF424490 UncPlanctomyceteAF513966 0.04 0.02 0.00 xxx 0.06 0.04 0.02 0.00 Pmy SOB1 SOB2 CLsulfateRedConAF029039 IsolAustOilAJ011042 UncBacteroidBacteriumAY133094 6A.BB B 23 CLColdMarineSedAJ240979 CYTOPHAGAsp.UncAJ240979 1A.BB B 74 CytophagaAB015525 CYTOPHAGAsp.AB015525 CLsulfaRedConAF029041 CLAntarcMarLakeAF142822 DCC CLAntMarLakeAF142822 CYTOPHAGAfermentansATCC19072 CYTOPHAGAfermentasATCC19072 CPHfermentans19072 CLHypersalineAlkalineLakeAF507 1A.BB B 1 CLAntarcMarLakeAF142901 CLAntMarLakeAF142901 1A.BB B 88 1A.BB B 31 3A.BB B 15 CFB 0.03 0.02 0.01 0.00 TCC SOB3
Sulphide oxidizing bacteria 1 CandidatusARCOBACTERsulfidicus 3A.LI B 70 STRJTB360 ARCOBACTERcryaerophilusCCUG178 CLAntarcContShelfSedAF424297 THIOMICROSPIRAdenitrificansDSM CLSB-17 STRNKB12 UncBacAF224804 Clhydrocarbon+seep+sediment CLhydrocarbonSeepSedAF154091 1A.LI B 41 ClanoxicZoneCariacoBasinAF2248 CLAnoxZCariacoBasAF224803 0.04 0.03 0.02 0.01 0.00
Deinecococcoides CLBacterioplArctOcAF355046 CLbacterioplArctOcAF355047 1A.LI B 11 CLAntarcContShelfSedAF424332 CLbacterioplankonAF001653 UncEubAB16-AF275926 2A.LI B 36 5A.LI B 90 CLAntarcMarLakeAF142828 CLHypersLakeLaysanHW-AF513964 DeinococcusRadiopugnansATCC191 CLchlorobenzeneCont.Groundwate DEHALOCOCCOIDESethenogenesAF00 CLt06f CLACE-22 UncVerrucomicrobiaAF424514 CLAnoxicMarineSedAY114335 CLanoxicMarineSediment 1A.LI B 68 UncBacAF275917 CLdeep-seaSedAB015533 CLvadinBE97 0.06 0.04 0.02 0.00
Sulfide oxidizing bacteria 3 1A.BB B 86 CLSAR193 UncAlphaAY033309 ACIDOCELLAspX91797 UncAlphaAF348719 SphingomonasMBIC3990 SPHINGOMONASyanoikuyaeX94099 HYPHOMONASjohnsonii SILICIBACTERlacuscaerulensisU7 strNKB7 CLDeepSeaSedToyamaBayAB077340 CLcoldMarineSedimentAJ240997 2A.BB B 43 CandidatusARCOBACTERsulfidicus 20A.BB B 16 STRJTB360 ARCOBACTERcryaerophilusCCUG178 CLAntarcContShelfSedAF424297 THIOMICROSPIRAdenitrificansDSM 1A.BB B 92 CLSB-17 STRNKB12 UncBacAF224804 ClanoxicZoneCariacoBasinAF2248 CLAnoxZCariacoBasAF224803 Clhydrocarbon+seep+sediment CLhydrocarbonSeepSedAF154091 0.08 0.06 0.04 0.02 0.00
Sulfur reducing ARCHEA 1A.BB+A+110 6A.BB+A+15 37A.BB+A+35 12A.BB+A+14 1A.BB+A+54 1A.BB+A+97 27A.BB+A+76 1A.BB+A+49 CLAfrGM CLAfrGoldMine3 CLAfrGoldMine1 UncArcSAGMA-G CLAfrGoldmine CLAfrGoldMine2 UncArcSAGMA-R Thermococcus mexicalis Termococcus waiotapuense Methanococcus infernus UncArcAF356638 0.06 0.04 0.02 0.00
Species 7A.LI B 64 SphingomonasMBIC3990 2A.LI B 88 SPHINGOMONASyanoikuyaeX94099 CLaquaticFormNullarborCavesAU 1A.LI B 107 UncUBA347051 UncAlphaAF348719 CLAntarcContShelfSedAF424318 ACIDOCELLAspX91797 2A.LI B 82 CLSAR193 UncPlanctomyceteAF424463 ALKALISPIRILLUMmobilisAF114783 UncAlphaAY033309 MarSnowAssCLagg27-L10943 HYPHOMONASjohnsonii UncBacAF328191 CandidatusARCOBACTERsulfidicus ThialkalivibrioDenitrifAF12654 SILICIBACTERlacuscaerulensisU7 PIRULELLAsp 3A.LI B 70 strNKB7 PLANCTOMYCESsp STRJTB360 UncGammaAF424060 CLDeepSeaSedToyamaBayAB077340 UncPlanctomyceteAF424462 ARCOBACTERcryaerophilusCCUG178 CLdeep-seaSedAB015544 CLcoldMarineSedimentAJ240997 CLAntarcContShelfSedAF424297 1A.LI B 81 UncCraterLakeAF316766 1A.LI B 14 THIOMICROSPIRAdenitrificansDSM CLAntarcContShelfSedAF424490 ECTOTHIORHODOSPIRAAF084511 CLSB-17 UncPlanctomyceteAF513966 0.04 0.02 0.00 STRNKB12 xxx 0.06 0.04 0.02 0.00 strBD3-6 UncBacAF224804 Pmy Clhydrocarbon+seep+sediment ThioplocaSp.Lake CLhydrocarbonSeepSedAF154091 1A.LI B 41 UncGammaAF424171 ClanoxicZoneCariacoBasinAF2248 CLAnoxZCariacoBasAF224803 SOB1 0.04 0.03 0.02 0.01 0.00 0.025 0.020 0.015 0.010 0.005 0.000 SOB2 CLBacterioplArctOcAF355046 CLbacterioplArctOcAF355047 1A.LI B 11 CLAntarcContShelfSedAF424332 CLbacterioplankonAF001653 UncEubAB16-AF275926 2A.LI B 36 5A.LI B 90 CLAntarcMarLakeAF142828 CLHypersLakeLaysanHW-AF513964 DeinococcusRadiopugnansATCC191 CLchlorobenzeneCont.Groundwate DEHALOCOCCOIDESethenogenesAF00 CLsulfateRedConAF029039 CLt06f IsolAustOilAJ011042 CLACE-22 UncBacteroidBacteriumAY133094 UncVerrucomicrobiaAF424514 6A.BB B 23 CLAnoxicMarineSedAY114335 CLColdMarineSedAJ240979 CLanoxicMarineSediment CYTOPHAGAsp.UncAJ240979 1A.LI B 68 1A.BB B 74 UncBacAF275917 CytophagaAB015525 CLdeep-seaSedAB015533 CYTOPHAGAsp.AB015525 CLvadinBE97 CLsulfaRedConAF029041 CLAntarcMarLakeAF142822 0.06 0.04 0.02 0.00 DCC CLAntMarLakeAF142822 CYTOPHAGAfermentansATCC19072 CYTOPHAGAfermentasATCC19072 CPHfermentans19072 CLHypersalineAlkalineLakeAF507 1A.BB B 1 CLAntarcMarLakeAF142901 CLAntMarLakeAF142901 1A.BB B 88 1A.BB B 31 3A.BB B 15 CFB 0.03 0.02 0.01 0.00 1A.BB B 86 CLSAR193 UncAlphaAY033309 ACIDOCELLAspX91797 UncAlphaAF348719 SphingomonasMBIC3990 SPHINGOMONASyanoikuyaeX94099 HYPHOMONASjohnsonii SILICIBACTERlacuscaerulensisU7 strNKB7 CLDeepSeaSedToyamaBayAB077340 CLcoldMarineSedimentAJ240997 2A.BB B 43 CandidatusARCOBACTERsulfidicus 20A.BB B 16 STRJTB360 ARCOBACTERcryaerophilusCCUG178 CLAntarcContShelfSedAF424297 THIOMICROSPIRAdenitrificansDSM 1A.BB B 92 CLSB-17 STRNKB12 UncBacAF224804 ClanoxicZoneCariacoBasinAF2248 CLAnoxZCariacoBasAF224803 Clhydrocarbon+seep+sediment SOB3 CLhydrocarbonSeepSedAF154091 0.08 0.06 0.04 0.02 0.00 IOB2 IOB3 IOB1
Iron Oxidizeing Bacteria 1 DEFERRIBACTERthermophilusU7560 DICTYOGLOMUSthermophilumX69194 CLGulfMexGasHydratesAY053495 CLGulfMexicoGasHydratesAY05349 1A.BB B 18 5A.BB B 7 5A.BB B 20 CLKTK32 CLbrineInt.ShabanDeepAJ347770 CLBrIntShabanDeepAJ347770 0.08 0.06 0.04 0.02 0.00
Iron Oxidizeing Bacteria 2 CLAnoxZCariacoBasAF224796 1A.LI B 19 ClColdmarineSedAJ240985 THIOBACILLUSferrooxidans UncDeltaAF348719 0.04 0.03 0.02 0.01 0.00
Species 7A.LI B 64 SphingomonasMBIC3990 2A.LI B 88 SPHINGOMONASyanoikuyaeX94099 CLaquaticFormNullarborCavesAU 1A.LI B 107 UncUBA347051 UncAlphaAF348719 CLAntarcContShelfSedAF424318 ACIDOCELLAspX91797 2A.LI B 82 CLSAR193 UncPlanctomyceteAF424463 ALKALISPIRILLUMmobilisAF114783 UncAlphaAY033309 MarSnowAssCLagg27-L10943 HYPHOMONASjohnsonii UncBacAF328191 CandidatusARCOBACTERsulfidicus ThialkalivibrioDenitrifAF12654 SILICIBACTERlacuscaerulensisU7 PIRULELLAsp 3A.LI B 70 strNKB7 PLANCTOMYCESsp STRJTB360 UncGammaAF424060 CLDeepSeaSedToyamaBayAB077340 UncPlanctomyceteAF424462 ARCOBACTERcryaerophilusCCUG178 CLdeep-seaSedAB015544 CLcoldMarineSedimentAJ240997 CLAntarcContShelfSedAF424297 1A.LI B 81 UncCraterLakeAF316766 1A.LI B 14 THIOMICROSPIRAdenitrificansDSM CLAntarcContShelfSedAF424490 ECTOTHIORHODOSPIRAAF084511 CLSB-17 UncPlanctomyceteAF513966 0.04 0.02 0.00 STRNKB12 xxx 0.06 0.04 0.02 0.00 strBD3-6 UncBacAF224804 Pmy Clhydrocarbon+seep+sediment ThioplocaSp.Lake CLhydrocarbonSeepSedAF154091 1A.LI B 41 UncGammaAF424171 ClanoxicZoneCariacoBasinAF2248 CLAnoxZCariacoBasAF224803 SOB1 0.04 0.03 0.02 0.01 0.00 0.025 0.020 0.015 0.010 0.005 0.000 SOB2 CLBacterioplArctOcAF355046 CLbacterioplArctOcAF355047 1A.LI B 11 CLAntarcContShelfSedAF424332 CLbacterioplankonAF001653 UncEubAB16-AF275926 2A.LI B 36 5A.LI B 90 CLAntarcMarLakeAF142828 CLHypersLakeLaysanHW-AF513964 DeinococcusRadiopugnansATCC191 CLchlorobenzeneCont.Groundwate DEHALOCOCCOIDESethenogenesAF00 CLsulfateRedConAF029039 CLt06f IsolAustOilAJ011042 CLACE-22 UncBacteroidBacteriumAY133094 UncVerrucomicrobiaAF424514 6A.BB B 23 CLAnoxicMarineSedAY114335 CLColdMarineSedAJ240979 CLanoxicMarineSediment CYTOPHAGAsp.UncAJ240979 1A.LI B 68 1A.BB B 74 UncBacAF275917 CytophagaAB015525 CLdeep-seaSedAB015533 CYTOPHAGAsp.AB015525 CLvadinBE97 CLsulfaRedConAF029041 CLAntarcMarLakeAF142822 0.06 0.04 0.02 0.00 DCC CLAntMarLakeAF142822 CYTOPHAGAfermentansATCC19072 CYTOPHAGAfermentasATCC19072 CPHfermentans19072 CLHypersalineAlkalineLakeAF507 1A.BB B 1 CLAntarcMarLakeAF142901 CLAntMarLakeAF142901 1A.BB B 88 1A.BB B 31 3A.BB B 15 CFB 0.03 0.02 0.01 0.00 1A.BB B 86 CLSAR193 UncAlphaAY033309 ACIDOCELLAspX91797 UncAlphaAF348719 SphingomonasMBIC3990 SPHINGOMONASyanoikuyaeX94099 HYPHOMONASjohnsonii SILICIBACTERlacuscaerulensisU7 strNKB7 CLDeepSeaSedToyamaBayAB077340 CLcoldMarineSedimentAJ240997 2A.BB B 43 CandidatusARCOBACTERsulfidicus 20A.BB B 16 STRJTB360 ARCOBACTERcryaerophilusCCUG178 CLAntarcContShelfSedAF424297 THIOMICROSPIRAdenitrificansDSM 1A.BB B 92 CLSB-17 STRNKB12 UncBacAF224804 ClanoxicZoneCariacoBasinAF2248 CLAnoxZCariacoBasAF224803 Clhydrocarbon+seep+sediment SOB3 CLhydrocarbonSeepSedAF154091 0.08 0.06 0.04 0.02 0.00 SRB1 DEFERRIBACTERthermophilusU7560 CLAnoxZCariacoBasAF224796 DICTYOGLOMUSthermophilumX69194 1A.LI B 19 CLGulfMexGasHydratesAY053495 ClColdmarineSedAJ240985 CLGulfMexicoGasHydratesAY05349 THIOBACILLUSferrooxidans 7A.LI B 30 1A.LI B 4 UncDeltaAF348719 3A.LI B 37 CLKTK32 0.04 0.03 0.02 0.01 0.00 CLbrineInt.ShabanDeepAJ347770 IOB2 SRB3 CLBrIntShabanDeepAJ347770 0.06 0.04 0.02 0.00 IOB3 SRB2 SRB4 SRB5 DEFERRIBACTERthermophilusU7560 DICTYOGLOMUSthermophilumX69194 CLGulfMexGasHydratesAY053495 CLGulfMexicoGasHydratesAY05349 1A.BB B 18 5A.BB B 7 5A.BB B 20 CLKTK32 CLbrineInt.ShabanDeepAJ347770 CLBrIntShabanDeepAJ347770 IOB1 0.08 0.06 0.04 0.02 0.00 SRB6 SRB7
Sulphate reducing bacteria 1 CLbrineInt.ShabanDeepAJ347761 CLBrIntShabanDeep 19A.LI B 50 11A.LI B 94 CLsaltmarsh CL+Delta CLdeltaProteobacteriaAY083025 1A.LI B 54 DESULFOMONASlimnicolaDSM2076 UncDeltaAF513951 STRHxd3 DESULFOBACTERIUMindolicumAJ237 DESULFOBOTULUS+U85470 CLOil-GroundwaterAB074951 DESULFOTIGNUMbalticumAF418176 2A.LI B 61 DESULFOSPIRAjoergenseniiX99637 CLCoastalAntarcticSedAY133462 CLcoastalAntarcticSedimentAY13 Desulfobacula+toluolica DESULFOBACULAtoluolicaAJ441316 0.06 0.04 0.02 0.00
Sulphate reducing bacteria 2 CLAntarcMarLakeAF142866 CLAntarctic+maritime 4A.LI B 16 CLFloodedRiceMicrocosmsAJ39046 THERMODESULFORHABDUSnorvegicus 0.04 0.03 0.02 0.01 0.00
CLAntarcMarLakeAF142849 UncBacAF419688 UncCFBAF424351 UncCFBAF424352 RIKENELLAmicrofususATCC29728 UncBacAF371922 CLArctOcBactplanktonAF354617 CLsulfateRedConAF029039 IsolAustOilAJ011042 UncBacteroidBacteriumAY133094 2A.LI B 1 2A.LI B 27 CLAntarcMarLakeAF142822 CLAntMarLakeAF142822 CytophagaAB015525 CYTOPHAGAsp.AB015525 CLsulfaRedConAF029041 CLColdMarineSedAJ240979 CYTOPHAGAsp.UncAJ240979 CLAntarcMarLakeAF142901 CLAntMarLakeAF142901 3A.LI B 86 CLHypersalineAlkalineLakeAF507 1A.LI B 71 CPHfermentans19072 CYTOPHAGAfermentansATCC19072 CYTOPHAGAfermentasATCC19072 0.04 0.03 0.02 0.01 0.00 Sulphate reducing bacteria 3
Sulphate reducing bacteria 4 CL+Delta CLdeltaProteobacteriaAY083025 DESULFOMONASlimnicolaDSM2076 UncDeltaAF513951 5A.BB B 39 STRHxd3 DESULFOBACTERIUMindolicumAJ237 0.020 0.015 0.010 0.005 0.000
Sulphate reducing bacteria 5 CLbrineInt.ShabanDeepAJ347761 CLBrIntShabanDeep 1A.BB B 58 CLsaltmarsh DESULFOBOTULUS+U85470 DESULFOTIGNUMbalticumAF418176 CLOil-GroundwaterAB074951 4A.BB B 41 CLCoastalAntarcticSedAY133462 CLcoastalAntarcticSed DESULFOSPIRAjoergenseniiX99637 Desulfobacula+toluolica DESULFOBACULAtoluolicaAJ441316 0.06 0.04 0.02 0.00
Sulphate reducing bacteria 6 CLbrineInterfaceSDeepAJ347756 CLBrIntShabanDeep 21U.BB B 27 2A.BB B 33 NEVSKIAramosaAJ001011 STRLX1 RHODANOBACTERlindaniclastLMG18 STENOTROPHOMONASmaltophilaAJ13 DESULFOBULBUSelongataDSM2908 DESULFOBULBUSrhabdoformisU1225 Desulfocapsa+sulfoexigens DESULFOFUSTISglycolicus 0.04 0.03 0.02 0.01 0.00
Sulphate reducing bacteria 7 CLAntarcMarLakeAF142866 CLAntarctic+maritime CLFloodedRiceMicrocosmsAJ39046 Desulfohalobium+retbaense DESULFOHALOBIUMretbaenseX99235 DESULFONATRIOVIBRIUMhydrognvrn DESULFONATRONOVIBRIUM sp STRTD3 1A.BB B 4 1A.BB B 52 1A.BB B 90 1A.BB B 59 2A.BB B 78 0.05 0.04 0.03 0.02 0.01 0.00
Methabolic limits ?! Chemistry Bacterial potentiality Basin Structure and Age 16S limits Possible Community Association Mathematic model