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ToxoDB. ApiDB Workshop June 2006. genetic markers. SAG4. Bs328. C810-A6. sourceDNA. end sequencing of small insert plasmid library. end sequencing of larger insert libraries (BAC/cosmid/plasmid). assembly. scaffold. physical gap. sequence gaps. Shotgun Sequencing Strategy.
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ToxoDB ApiDB Workshop June 2006
genetic markers SAG4 Bs328 C810-A6 sourceDNA end sequencing of small insert plasmid library end sequencing of larger insert libraries (BAC/cosmid/plasmid) assembly scaffold physical gap sequence gaps Shotgun Sequencing Strategy
Sequencing Scaffolds Pinned to the Genetic Map: chromosomes 1a & 1b (Sanger) whole genome shotgun (TIGR) unmapped markers (telomeric?) mapping ambiguities Sequencing & assembly: Ian Paulsen, Neil Hall Genetic map: Chunlei Su & David Sibley Mapping: Bindu Gajria & David Roos
Improved Genetic & Physical Maps A. Khan et al (2005) Nucl. Acids Res. 33:2980