INTRODUCTION

1. ISO 29 ISO 25 ISO 27 A B C ANTIMICROBIAL EFFECTS OF AQUEOUS AND GEL CHLORHEXIDINE C.C.P.GUIMARÃES1,2, S. CAI3, J.L.LAGE-MARQUES2 Endodontics - University Santo Amaro1; University of São Paulo2; ICB-USP3 - Brazil 3325 clauvinajp@uol.com.br INTRODUCTION When growth occurred it was checked in every instance for purity of E. Faecalis Blocks were mounted in supports Antimicrobial dressings have been used as intracanal medications for many years although the ideal formula has not been established. Besides some microorganisms are more resistant than others to root canal therapy. The aim of the present study was to evaluate if different vehicles enhance the antimicrobial efficacy of chlorhexidine against Enterococcus faecalis. Dentin blocks were sterilized (for 15 min at 121ºC ) They were infected with a standard suspension of E. faecalis ATCC 29212 GROUP I - 2% aqueous chlorhexidine (n=3) MATERIALS AND METHODS UFC of E. faecalis in TSA plates Smear of E. faecalis in Gram stain Blocks were divided into groups for application of medications GROUP II - 2% chlorhexidine with natrosol gel as vehicle (n=3) Bovine incisors prepared in discs All procedures were repeated six times GROUP III - control (saline) (n=2) RESULTS Canals were widened with an ISO 23 round bur Incubation for 24 hr at 37 ºC Group I 36 36 36 36 36 36 Group II Scanning electron microscopic view from smeared specimen (bovine dentin) after the round bur use Group III Dentin chips were taken with sterile round burs of crescent sizes in tubes with TSB 24 24 24 24 24 24 Total tubes Number of tubes 2 2 3 5 4 3 ISO 25 27 29 25 27 29 25 27 29 Results were analyzed using the chi-square test. The smear layer was removed by immersion in 17% EDTA-T solution in a ultrasonic bath for 5 min followed by 0,5% NaOCl for 5 min CONCLUSION Incubation for 24 hr at 37 ºC Both medications were equally effective against E. faecalis with no statistically significant differences between them. Scanning electron microscopic view from a specimen (bovine dentin) after the ultrasonic bath Afterwards, two new tubes were inoculated with 200 L of each tube (A, B and C) * Supported by University of Santo Amaro (UNISA)