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Are there Microbes Lurking?

June 12, 2012 | State University of NY. Paul W. Tranchell CIH, CSP, RBP Project Manager. Are there Microbes Lurking?. June 12, 2012 | State University of NY. Are there Microbes Lurking?. Industrial Hygiene. Occupational Stressors: (i.e. biological agents). Anticipation Recognition

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Are there Microbes Lurking?

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  1. June 12, 2012 | State University of NY Paul W. Tranchell CIH, CSP, RBP Project Manager Are there Microbes Lurking?

  2. June 12, 2012 | State University of NY Are there Microbes Lurking?

  3. Industrial Hygiene Occupational Stressors: (i.e. biological agents) • Anticipation • Recognition • Evaluation • Control

  4. Agenda Definition Normal Locations Selected Suppliers Unusual & Ancillary Biohazards Biosafety Fundamentals

  5. Biosafety • The application of knowledge, techniques and equipment to prevent personal, laboratory, and environmental exposure to potentially infectious agents or biohazards • Biosafety defines the containment conditions under which infectious agents can be safely manipulated • The objective of containment is to confine biohazards and to reduce the potential exposure of the laboratory worker, persons outside of the laboratory, and the environment to potentially infectious agents • Medicine.net http://www.medterms.com/script/main/art.asp?articlekey=33817

  6. Normal Locations • Bloodborne Pathogens • Microbiological Laboratories • Animal Laboratories • Molecular Biology Labs

  7. Select Suppliers • American Type Culture Collection (ATCC) - Repository of a huge number of cell lines • CABRI (Common Access to Biological Resources and Information) • Developmental Studies Hybridoma Bank - Monoclonal antibodies • DSMZ - The DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH = German Collection of Microorganisms and Cell Cultures) is a non-profit public domain general repository specializing in tumor cell lines. It holds the largest collection of human hematopoietic cell lines currently available worldwide. • European Collection of Cell Cultures (ECACC) - ECACC "was established in 1984 as a cell culture collection to service the research community and provide an International Depository Authority recognised patent depository for Europe." • HyperCLDB - Hypertext resource derived from the Cell Line Data Base (CLDB) with descriptions of more than 4,000 cell lines • National Cell Culture Center - A "national resource facility established by the National Institutes of Health (NIH) to provide customized, large scale, cell culture services for basic research laboratories."

  8. Unusual & Ancillary Hazards • Field Work • Lyme Disease • Insect Bites • Suborder Surpentes • Rodent Diseases • Plague • Hanta Virus • HVAC / Legionella • Bird Droppings (no bleach please) • Mold • Suspicious Packages / ”white” powder • Drum Skins

  9. Biosafety Fundamentals Chain of Infection Risk Assessment Reservoir of pathogen Portal of escape Transmission Route of entry / infectious dose Susceptible host Incubation period Facilities Practices / Equipment Access control / Immunization PPE Surveillance

  10. Biosafety Fundamentals Definitions kcd11/HE.prn/NYCER-Biosafety/NYCER_BSL2&3-121311.pptx

  11. Biosafety Fundamentals Control Strategies • Standard Microbiological Practices • Special Microbiological Practices • Safety Equipment • Primary Barrier • Biological Safety Cabinets (BSC) • Types, Selection, Use! • Personal Protective Equipment • Laboratory Facilities • Secondary Barriers kcd11/HE.prn/NYCER-Biosafety/NYCER_BSL2&3-121311.pptx

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