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SURELY BETTER

SURELY BETTER. PCR REAGENTS. AND INSTRUMENTS. Table of Contents. The ArchaeMaxx Polymerase –Enhancing Factor ArchaeMaxx overcomes the stalling of proofreading enzymes when dUTP is present within the template or PCR reaction. Exclusively found in Agilent’s high-fidelity DNA polymerases.

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SURELY BETTER

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  1. SURELY BETTER PCR REAGENTS AND INSTRUMENTS

  2. Table of Contents

  3. The ArchaeMaxxPolymerase –Enhancing Factor ArchaeMaxx overcomes the stalling of proofreading enzymes when dUTP is present within the template or PCR reaction. Exclusively found in Agilent’s high-fidelity DNA polymerases. Fusion Technology The processivity of Agilent’s premium enzymes are dramatically increased by fusing the DNA polymerase with a high-affinity double-stranded DNA binding domain. High-Fidelity HotStart Formulation Master Mix Format

  4. Polymerase Selection Guide High Fidelity & Difficult/GC-Rich PCR Specialty Enzymes Routine Enzymes

  5. Surely better PCR functionality for your lab SureCycler 8800 The SureCycler 8800 quickly runs complex techniques with superior gradient and uniformity, volume flexibility, touch screen interface, and a PCR protocol wizard. Gradient Gradient enables single run optimization of your protocol Uniformity Market leading uniformity gives superior results every time you run your protocol

  6. One easy decision to a complete portfolio of PCR solutions SureCycler 8800

  7. Extreme accuracy – 1 error/2.5 million bp PfuUltra II HotStart DNA Polymerase When accuracy is critical for your downstream applications, such as cloning, protein crystallization studies, and sequencing, PfuUltra II is the industry standard for high-fidelity PCR. • Highest fidelity for the most reliable, error free PCR • 70-80% quicker time to results • Less than 0.5% errors in full length 500 bp PCR products • Accurately amplifies targets up to 19 kb genomic DNA • Average error rate 3x lower than PfuTurbo & 20x lower than Taq Fidelity measured using Agilent’s validated and reference fidelity assay. (Accuracy = 1/error rate)

  8. Superior yield with extension rates of 15 sec/kb Herculase II Fusion DNA Polymerase Herculase IIhelps you overcome challenging PCR targets with the combination of exclusive enzyme improvement additives and an optimized buffer system, allowing you to easily amplify targets with GC-content as high as 84%. • Superior yield for routine PCR applications & difficult/GC-rich targets • High sensitivityfor amplification of low amounts of DNA • Fast cycling timeswith 15 sec/kb extension rate • Accurately amplifies targets up to 23 kb genomic DNA • Preserves Pfu fidelity1 error/770,000 bases Herculase II easily amplifies GC-rich fragments from human genomic DNA: IGFB, human insulin-like growth factor (79% GC); Fragile X metal retardation syndrome protein (84% GC); HTR, hyrdroxytryptomine receptor C2 fragment (65% GC); MMZ5, AIC5-zince family member 5 protein (68% GC)

  9. Economical hot start DNA polymerase Paq5000 DNA Polymerase Paq5000 is capable of quickly and robustly amplifying DNA targets up to 6 kb genomic DNA. With a shortened extension rate of 30 sec/kb, compared to 1 min/kb for Taq DNA polymerase, Paq5000 minimizes the overall cycle time. • Easily replaces Taq in standard PCR protocols with minimal optimization • Pre-optimized reaction buffers included • Fast cycling timeswith 30sec/kb extension rate Amplification of human α-1 anti-trypsin gene and 6 kb fragment of human ß-gene using Paq5000 HotStart PCR Master Mix and the closest hot start Taq master mix competitor.

  10. Overcome uracil stalling PfuTurbo Cx HotStart DNA Polymerase PfuTurbo Cx is formulated with a novel mutant of Pfu DNA polymerase that completely overcomes uracil stalling without sacrificing fidelity. PfuTurbo Cx reads through dUTP located in both the template strand and extending strand. • World’s only high-fidelity polymerase that efficiently reads through dUTP • Great for bi-sulfite sequencing, UNG decontamination protocols, and DNA methylation studies • Amplifies templates 5-10 kb or with high GC-content • DMSO provided separately to assist in optimizing PCR conditions

  11. One-step high-fidelity TA/UA PCR cloning Low copy number target amplification in complex samples Easy-A High-Fidelity PCR Cloning Enzyme Easy-A is the only proofreading DNA polymerase that delivers your PCR products ready for high-throughput TA/UA cloning with greater that 95% cloning efficiency. PicoMaxx High-Fidelity PCR System PicoMaxx possesses the sensitivity to detect low copy number targets and the robustness to withstand the effects of PCR inhibitors without the need for extensive purification and subsequent DNA loss.

  12. Ensure you achieve successful amplification PCR Enhancers Perfect Match PCR Enhancer • Reduces PCR artifacts by increasing specificity • Destabilizes mismatched primer-template complexes Taq Extender PCR Additive • Improves length, yield, and reliability of PCR with Taq DNA polymerase • Enhances the efficiency of template extension by Taq DNA polymerase

  13. Highest reverse transcription accuracy Complete system for high-fidelity RT-PCR AccuScript High-Fidelity Reverse Transcriptase AccuScript reduces errors during cDNA synthesis with 3.7x more accuracy than other commercially available RTs and 6.6x more accuracy than AMV RT. AccuScript PfuUltra II RT-PCR Kit The AccuScript PfuUltra II RT-PCR Kit combines the accuracy of AccuScript and PfuUltra II to provide you a complete system for RT-PCR in applications that demand high-fidelity, specificity, and throughput.

  14. Broad activity in temperatures ranging from 37-55° C High yield single-tube RT-PCR AffinityScript Multiple Temperature Reverse Transcriptase AffinityScript assures reverse transcription through GC-rich sequences with cDNA yields, whether priming at room temperature with random hexamers or at stringent temperatures to enhance specificity. AffinityScript One-Step RT-PCR Kit The AffinityScript One-Step RT-PCR Kit tolerates higher concentrations of inhibitors such as guanidine isothiocyanate, ethanol, formamide, and pectin for improved results.

  15. Fast and reliable cloning Rapid plasmid purification StrataClone PCR Cloning Kit The StrataClone PCR Cloning Kit allows high-efficiency 5-minute cloning of PCR products, using the DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase. StrataPrep Plasmid Miniprep Kit The StrataPrep Plasmid Miniprep Kit provides a rapid, phenol-free method for purifying pure plasmid DNA from bacterial cultures.

  16. Produces ready-to-use highly purified DNA Purifies DNA fragments ranging from 100 bp to 23 kb StrataPrep PCR Purification Kit The StrataPrep PCR Purification Kitprovides a rapid silica-based method for separation of PCR products from PCR primers, unincorporated nucleotides, buffer components and enzymes. StrataPrep DNA Gel Extraction Kit The StrataPrep DNA Gel Extraction Kitallows rapid, high-yield isolation of pure DNA from agarose gels (up to 10 μg).

  17. Labware designed with you in mind StrataCoolers StrataCooler LP Benchtop Cooler Provides the most protection for your enzymes whether in your freezer or on your benchtop, maintaining -15° C for at least 2 hours. StrataCooler Cryo Preservation Module Enables controlled freezing (0.4-0.6° C/min) of cells to significantly increase survival rates (up to 90%)

  18. Dedicated to meeting all of your life science needs Agilent Custom Manufacturing Services Whether you need bulk packaging, custom formulations, higher-level documentation, or want to establish an OEM relationship, Agilent’s dedicated team of experts will help you find the fastest, most cost-effective way to reach your goals. • Area of Expertise • Bulk and custom manufacturing • Enzymes, PCR, QPCR and reagent blends • Competent cells • Protein expression • RNA, DNA & protein purification Custom packaging and labeling Materials management Quality management and documentation

  19. Start-to-finish PCR solution Agilent Combination Packs The combination packs, designed to provide a start-to-finish solution with your thermal cycler, simplifies your ordering process while delivering the highest performing technologies. In addition to all required reagents, each combination pack includes PCR plasticware and a StrataCooler to ensure you have ever you need all in one easy ordering step. • General PCR Combo Pack • Cloning PCR Combo Pack • Site-Directed Mutagenesis Combo Pack • General RT-PCR Combo Pack • Genotyping PCR Combo Pack For kit components click here

  20. For more information, please visit www.agilent.com/genomics/pcr Follow us on Twitter & YouTube @AgilentLife http://www.youtube.com/AgilentLife

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