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Fun DNA Facts and Extraction Methods Presentation

Explore the fascinating world of DNA with fun facts and learn about DNA extraction methods from plants. Discover how DNA determines all living organisms' characteristics and the structure of DNA.

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Fun DNA Facts and Extraction Methods Presentation

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  1. Class URL: http://stan.cropsci.uiuc.edu/courses/index.php For powerpoints and handouts. Let me know if there’s a problem. Also, please drop me an email if you’d like to come to office hours (anytime, by appointment).

  2. Waiter! There’s DNA in my food!

  3. Fun DNA fact #1 • The information in the human genome would fill two hundred 500-page telephone directories. • The genomes of most commonly grown crops (corn, soybean) are about as large, or larger (wheat).

  4. DNA – blueprint of life • DNA determines the characteristics of all living organisms. Well, unless you count viruses. • DNA is composed of a four-letter nucleotide/molecule alphabet referred to as A, T, C, and G. • The combination of letters determines all the inherited characteristics of the living organism. • Each cell in the human body contains >3 BILLION letters. A soybean cell, >1.2 billion.

  5. (Franklin), Crick, Watson (and Wilkins) – structure of DNA

  6. A double helix with two antiparallel strands

  7. “It has not escaped our notice that the specific pairing we have postulated immediately suggests a copying mechanism for the genetic material.”

  8. Base Pairing

  9. 5’ 3’

  10. Nucleus (DNA) Chloroplast Vacuole Mitochondrion Cytoplasm Cell Wall Cell Membrane

  11. DNA extraction from plants • Smush (“homogenize”) the cells • Dissolve membranes and solublize DNA in a detergent buffer. • Remove solids with centrifugation • Remove proteins with phenol extraction • Precipitate DNA with alcohol

  12. Fun DNA fact #2 Each cell contains 9 feet of DNA. In an average meal, you eat approximately 55,000,000 cells or about 93,205 miles of DNA.

  13. DNA extraction issues • Homogenization - has to be “just right” • RNA • Hydrodynamic shearing - treat it gently

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