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Class URL:. http://stan.cropsci.uiuc.edu/courses/index.php For powerpoints and handouts. Let me know if there ’ s a problem. Also, please drop me an email if you ’ d like to come to office hours (anytime, by appointment). Waiter! There ’ s DNA in my food!. Fun DNA fact #1.

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  1. Class URL: http://stan.cropsci.uiuc.edu/courses/index.php For powerpoints and handouts. Let me know if there’s a problem. Also, please drop me an email if you’d like to come to office hours (anytime, by appointment).

  2. Waiter! There’s DNA in my food!

  3. Fun DNA fact #1 • The information in the human genome would fill two hundred 500-page telephone directories. • The genomes of most commonly grown crops (corn, soybean) are about as large, or larger (wheat).

  4. DNA – blueprint of life • DNA determines the characteristics of all living organisms. Well, unless you count viruses. • DNA is composed of a four-letter nucleotide/molecule alphabet referred to as A, T, C, and G. • The combination of letters determines all the inherited characteristics of the living organism. • Each cell in the human body contains >3 BILLION letters. A soybean cell, >1.2 billion.

  5. (Franklin), Crick, Watson (and Wilkins) – structure of DNA

  6. A double helix with two antiparallel strands

  7. “It has not escaped our notice that the specific pairing we have postulated immediately suggests a copying mechanism for the genetic material.”

  8. Base Pairing

  9. 5’ 3’

  10. Nucleus (DNA) Chloroplast Vacuole Mitochondrion Cytoplasm Cell Wall Cell Membrane

  11. DNA extraction from plants • Smush (“homogenize”) the cells • Dissolve membranes and solublize DNA in a detergent buffer. • Remove solids with centrifugation • Remove proteins with phenol extraction • Precipitate DNA with alcohol

  12. Fun DNA fact #2 Each cell contains 9 feet of DNA. In an average meal, you eat approximately 55,000,000 cells or about 93,205 miles of DNA.

  13. DNA extraction issues • Homogenization - has to be “just right” • RNA • Hydrodynamic shearing - treat it gently

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