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RISK Categories

RISK Categories. Risk group vs biosafety level Both 1, 2, 3 and 4 not always the same. Biosafety Levels. Basic - Biosafety Level 1 , Basic – Biosafety Level 2 , Containment – Biosafety Level 3, and Maximum Containment – Biosafety Level 4.

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RISK Categories

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  1. RISK Categories • Risk group vsbiosafety level • Both 1, 2, 3 and 4 not always the same

  2. Biosafety Levels • Basic - Biosafety Level 1 , Basic – Biosafety Level 2, Containment – Biosafety Level 3, and Maximum Containment – Biosafety Level 4. • Biosafety level designations are based on a composite of the: • design features, • construction, • containment facilities, • equipment, • practices and operational procedures

  3. General Laboratory Safety Procedures • Be familiar with the materials you are working with (e.g., chemical, biological, radioactive). Written laboratory protocols and review the MSDS (Material Safety Data Sheets) for chemicals. Consider the toxicity of the materials and the health and safety hazards of each procedure (e.g., generation of aerosols).  Take advantage of the knowledge and experience of laboratory personnel and the safety equipment that is available. • Know the location of safety equipment and emergency procedures in your area. • Always wear appropriate clothing (e.g., pants, shirts, shoes) in the laboratory. Open sandals are prohibited; shorts and skirts are not recommended. • Do not work alone in the laboratory. When hazardous operations are conducted, arrangements should be made to have another person present in the lab. • Keep the laboratory and work area clean and uncluttered. • Work with all hazardous chemicals inside a fume hood. • Never eat, smoke, drink, prepare food or apply cosmetics in the laboratory. • Do not leave reactions unattended. • Prohibit unauthorized individuals from entering the laboratory.  

  4. GMT: Good Microbiological Techniques • using proper aseptic techniques; • wearing appropriate personal protective equipment;  • minimizing the production of aerosols or working in a biological safety cabinet when aerosol production is unavoidable;  • employing universal precautions with bloodborne pathogens; • routinely disinfecting work surfaces and equipment/supplies used in the experiment; • immediately cleaning spills according to laboratory protocols; • restricting work with infectious material to one area of the laboratory; • properly disposing of waste; • routinely washing hands following any experiment and after removing gloves; and • reporting all accidents/incidents and seeking medical attention if necessary.

  5. How do you decide? • Pathogenicityof the organism. • Mode of transmission and host range of the organism. • Local availability of effective preventive measures. These may include: prophylaxis by immunization or administration of antisera (passive immunization); sanitary measures, e.g. food and water hygiene; control of animal reservoirs or arthropod vectors. • Local availability of effective treatment. This includes passive immunization, postexposurevaccination and use of antimicrobials, antivirals and chemotherapeutic agents, and should take into consideration the possibility of the emergence of drug-resistant strains.

  6. Risk Assesment • What are you working with? • Microbe (risk category) • Chemical (MSDS) • Equipment (?)

  7. Biosafety in Pakistan

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