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Meristem culture of Actinidia sp. Authors names: Anca Barcu. Coordinator Professor: Adrian Peticila. International Student Symposium "Hortus Academicus“ March 29, 2019 - Bucharest, Romania. Introduction. Actinia deliciosa (A) Actinida chinensis (C) Actinidia arguta (D).
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Meristemculture of Actinidiasp. Authors names: Anca Barcu Coordinator Professor: Adrian Peticila International Student Symposium "Hortus Academicus“ March 29, 2019 - Bucharest, Romania
Introduction • Actinia deliciosa (A) • Actinidachinensis (C) • Actinidiaarguta (D) International Student Symposium "Hortus Academicus“ March 29, 2019 - Bucharest, Romania
Materials and Methods • Meristem culture • 52 meristems • 4 types of medium International Student Symposium "Hortus Academicus“ March 29, 2019 - Bucharest, Romania
Results and discussions • -remarkable growth on medium with IBA, GA3 and ABA, 3 days dark > 7 days dark • -high viability on the same medium • -viable meristems after 5 days in sterilized water vs 7 days in sterilized water at 5C International Student Symposium "Hortus Academicus“ March 29, 2019 - Bucharest, Romania
Conclusions and recommendations • -the best medium for growth is also the best medium for inoculation • -actinidiaargutagrows much faster than chinensis and deliciosa International Student Symposium "Hortus Academicus“ March 29, 2019 - Bucharest, Romania
References • Micropropagation of kiwifruit using non-axenic shoot tips, PAUL L. MONETTE • Akbaş, Filiz & Işıkalan, Çiğdem & Namli, Süreyya & Başaran, Davut. (1560). Micropropagation of kiwifruit (Actinidiadeliciosa). INTERNATİONAL JOURNAL OF AGRİCULTURE & BİOLOGY. 8530. 9-3. • Micropropagation of Kiwifruit (Actinidiadeliciosa) FİLİZ ADIYAMAN AKBAŞ, ÇİĞDEM IŞIKALAN, SÜREYYA NAMLI1 AND DAVUT BAŞARAN • Rugini E., Gutiérrez-Pesce P. (2003) Micropropagation of Kiwifruit (Actinidia spp.). In: Jain S.M., Ishii K. (eds) Micropropagation of Woody Trees and Fruits. Forestry Sciences, vol 75. Springer, Dordrecht • Revilla M.A., Rey M.A., Gonzalez-Rio F., Gonzalez M.V., Diaz-Sala C., Rodriguez R. (1992) Micropropagation of Kiwi (Actinidia spp.). In: Bajaj Y.P.S. (eds) High-Tech and Micropropagation II. Biotechnology in Agriculture and Forestry, vol 18. Springer, Berlin, Heidelberg • Wessels E, Nel DD, Von Staden DFA (1984) In vitro propagation of Actinidiachinensis PI. Cultivar Hayward. Deciduous Fruit Grower 34: 453–457 • Tsai C-K (1988) Plant regeneration from leaf callus protoplasts of ActinidiachinensisPlanch, var. chinensis. Plant Sci 54: 231–250 International Student Symposium "Hortus Academicus“ March 29, 2019 - Bucharest, Romania