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Intro to the Lab

This talk introduces the lab and provides tips on staying safe, organized, and happy. Topics include lab events, resources, lab safety, staying organized, and lab etiquette.

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Intro to the Lab

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  1. Intro to the Lab Erin Osborne Nishimura June 5, 2017

  2. Today’s talk • Welcome to the lab • Staying safe in the lab • Staying organized in the lab • Staying happy in the lab

  3. Welcome to the lab

  4. Lab events • Lab meetings • Wednesdays at 11am, starting July 5th • Everyone expected to attend • Standing meetings • Journal clubs • Fridays at 3pm, starting June 30 • Grads, senior scientists expected to attend • Undergrads and volunteers optional but encouraged • Upcoming Symposia • Q-Bio, GAUSSI, Biochemistry Department Retreat, Rocky Mountain Computing, IGAF

  5. Digital Lab Resources • Lab webpage • Onishlab.colostate.edu • Onishlab.colostate.edu/log-in  password: celltype227 • Pangea • Inventories, protocols, purchases, lab meetings • Quartzy • ordering • EON-NAS • Data, images, sequencing, & backups • LabArchives

  6. Staying safe in the lab

  7. Chemicals and hazardous materials • Receive proper training • Stay informed about chemicals and their toxicity • Use appropriate personal protective equipment • Lab coat • Lab goggles • Wear appropriate clothes • Close-toed shoes • Full length pants • Long hair tied back • Use fume hood for hazardous and stinky chemicals • Phenol, chloroform, formamide, formaldehyde, etc. • Label all chemicals properly • Full names (ethanol) • Hazardous chemicals must be in cabinets and secondary containers

  8. Hazardous waste disposal • Secondary containers • Weekly inspections • Disposal after 90 days • Chemical solid waste

  9. Other lab hazards • Glass • Razors • Keep in styrofoam • Avoid using excessive force • Liquid nitrogen, dry ice • Exploding tubes • Centrifuges • Always balance • Microscope light sources • Gel Red • Don’t touch it

  10. Leaving the lab at the end of the day • Turn off all bunsen burners & other flames • Turn off all heated stir plates & heated equipment • Turn off all microscope lamps • Check around if you are the last person to leave

  11. Staying organized in the lab

  12. Stay organized in the lab • Follow protocols • “Your protocol must be in your hand” –Kohta Ikegami • Take notes • Date everything • Keep personal inventories • wEN001 – Erin’s worms • pEN001 – Erin’s plasmids • oEN001 – Erin’s oligos • Contribute to lab inventories

  13. Lab notebook • Everyone must have a paper lab notebook • Digital Lab notebook is available for regular lab members through LabArchives

  14. Digital files – backing up • 3 copies across two locations • Work locally AND backup to EON-NAS (RAID5, 30-day versioning) • OR work on EON-NAS and backup locally • Third location • currently up to you • Options coming soon • Automated sync

  15. Staying happy in the lab

  16. Being courteous • Noise & talking • Headphones & music • Cleaning up • Do we need lab chores? • Shared jobs • __ Autoclave waste • __ dishwashing • __ tip & tube autoclaving • __ LB & NGM • __ spatulas (buy more) • __ M9 & liquid LB • __ Antibiotic box

  17. Being hygienic • Enzymes in coolers • Sterile, clean technique when using stocks and enzymes • No double gloves in the hallways

  18. Re-order! • Use quartzy • onishimuralab@lists.colostate.edu

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