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Living cerebral endothelial cells

Explore the effects of mannitol treatment on cerebral endothelial cells using atomic force microscopy. Investigate changes in cell morphology and adhesion properties before and after treatment for potential insight into blood-brain barrier function.

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Living cerebral endothelial cells

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  1. Living cerebral endothelial cells György Váró Lamorlaye, May 2011 Institute of Biophysics, Biological Research Center Szeged, Hungary

  2. Mannitol treatment of the cerebral endothelial cells Blood-brain barrier: Mannitol: HO - CH2 – (CHOH)4 – CH2 - OH

  3. After treatment Before treatment

  4. Before treatment After treatment

  5. A M S C 0.6 0.4 force (nN) 0.2 0 -600 -400 -200 200 0 distance (nm) B 0.6 M C 0.4 force (nN) 0.2 0 200 400 0 600 indentation (nm) 8 kPa 0.9 kPa

  6. Z. Bálint, I.a. Krizbai, I. Wilhelm, A.E. Farkas, Á. Párducz, Z. Szegletes and G. Váró (2007) Changes induced by hyperosmotic mannitol in cerebral endothelial cells: an atomic force microscopic study. Eur. Biophys. J. 36. 113-120

  7. Effect of the calcium starvation on the cells: Healthy endothelial cells Ca starved cells -Ca2+

  8. Ca starved cells +Ca2+

  9. I. Wilhelm, A.E. Farkas, P. Nagyőszi, G. Váró, Z. Bálint, G.A. Végh, P.O. Couraud, I.A. Romero, B. Weksler and I.A. Krizbai (2007) Regulation of cerebral endothelial cell morphology by extracellular calcium. Phys. Med. Biol. 52. 6261-6274

  10. Transmigration of the melanoma cells AFM cantilever A2058 RBEC Layer 8 µm Filter Matrigel Petri Dish

  11. A2058 on RBEC layer

  12. 3

  13. Adhesion force between cells

  14. AFM cantilever tip on hard surface Petri dish

  15. AFM cantilever tip on hard surface dwell time load

  16. AFM cantilever tip on living cells

  17. AFM cantilever tip on living cells Stress relaxation

  18. AFM tipless cantilever with melanoma cell on living cells

  19. AFM tipless cantilever with melanoma cell on living cells

  20. AFM tipless cantilever with melanoma cell on living cells AFM tipless cantilever with melanoma cell on fixed cells

  21. Adhesion force: Melanoma cell on hard surface Melanoma cell on living cell layer Melanoma cell on fixed cell layer

  22. Stress relaxation force Melanoma cell on hard surface Melanoma cell on living cell layer Melanoma cell on fixed cell layer

  23. Adhesion force properties: Numbers of jumps Size of the jumps

  24. Size of the jumps Living cells Fixed cells

  25. Stress relaxation fast exp.

  26. Stress relaxation slow exp.

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