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Figure 16.1 and 6

Figure 16.1 and 6. James Watson and Francis Crick. Rosalind Franklin and her X-ray diffraction photo of DNA.

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Figure 16.1 and 6

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  1. Figure 16.1 and 6 James Watson and Francis Crick Rosalind Franklin and her X-ray diffraction photo of DNA.

  2. >gi|14456711|ref|NM_000558.3| Homo sapiens hemoglobin, alpha 1 (HBA1), mRNA ACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGTGGCCATGCTTCTTGCCCCTTGGGCCTCCCCCCAGCCCCTCCTCCCCTTCCTGCACCCGTACCCCCGTGGTCTTTGAATAA AGTCTGAGTGGGCGGC LE 16-5 http://www.ncbi.nlm.nih.gov/

  3. (a) Space-fillingmodel Key features ofDNA structure (c) 5 end G C Hydrogen bond C G 3 end C G C A T G 3.4 nm Figure 16.7 A T C G C G C G A T 1 nm G C T A C G C G T A G C 3 end A T A T 0.34 nm 5 end A T (b) Partial chemical structure

  4. Sugar Figure 16.8 Sugar Adenine (A) Thymine (T) Sugar Sugar Guanine (G) Cytosine (C)

  5. Figure 16.UN04

  6. Purine  purine: too wide Figure 16.UN01 Pyrimidine  pyrimidine: too narrow Purine  pyrimidine: widthconsistent with X-ray data

  7. Stability of DNA: example of a DNA melting curve. UV absorbance spectra of native and heat-denatured E. coli DNA. Voet, Fundamentals, 2nd

  8. Variation of the melting temperatures, Tm, of various DNAs with their G + C content. Relevance for DNA melting and hybridization PCR (polymerase chain reaction) DNA microarrays (gene expression) Helicases, polymerases (transcription, duplication) Chargaff’s rule shows correlation between Tm and G+C content Voet and Voet, Biochemistry, chapter 5

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