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Genetics News. EditBase. Mystery Sequence. EditBase format. Pure text format. Problem Set 4. Study Question 3. What is the minimum number of bases to specify the number of different amino acids found in protein?. 1 base fixed length nonoverlapping. G A C T. Study Question 3.
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Genetics News EditBase Mystery Sequence EditBase format Pure text format Problem Set 4
Study Question 3 What is the minimum number of bases to specify the number of different amino acids found in protein? 1 base fixed length nonoverlapping G A C T
Study Question 3 What is the minimum number of bases to specify the number of different amino acids found in protein? 2 base, fixed length, nonoverlapping
Study Question 3 What is the minimum number of bases to specify the number of different amino acids found in protein? 3 base, fixed length, nonoverlapping
Growth of E. coli B + phage T4 Seed plate withE.coli B andphage T4(not visible)
rII region of phage T4 T4 rII+ T4 rII - T4 DNA T4 DNA rII rII rIIA rIIB rIIA- rIIB
Distinguishing T4 rII+from rII- T4 rII+T4 rII - T4 rII+T4 rII - E. coli B x E. coli K() x T4 rII - cannot grow on E. coli K()
Why is T4rII-defective? T4 rII - E. coli K() No infection T4 rII+ Lysis E. coli K()
Crick et al frameshift experiment rIIA+ proflavin rIIA- proflavin rIIA- proflavin rIIA-
How to explain frameshift results?Role of proflavin in experiment G T G A G C C A C T C G G A T A G T G A G C A C C A C T C G ?? G A T A Proflavin causes one base insertions (or deletions)
How to explain frameshift results?Wild type rIIA rIIA+ Wld type rIIA … THE FAT CAT ATE THE BIG RAT...
How to explain frameshift results?Single frameshift rIIA- … THE FAT CAT ATE THE BIG RAT... THE FAT CAR TAT ETH EBI GRA …
How to explain frameshift results?Double frameshift rIIA- … THE FAT CAT ATE THE BIG RAT... … THE FAT CAR TAT ETH EBI GRA … … THE FAT CAR TAR TET HEB IGR …
How to explain frameshift results?Triple frameshift rIIA- … THE FAT CAT ATE THE BIG RAT... … THE FAT CAR TAT ETH EBI GRA … … THE FAT CAR TAR TET HEB IGR … … THE FAT CAR TAR TOE THE BIG ...
Study Question 7 When will proflavin-induced mutation suppress?
Study Question 8 Results of frameshift experiment if variable length code? This is a variable length code Variable length code with GATC? This is a varitable length code