Definition

1. USBOT-403- Semi - IVAnther cultureDr. L. R. RathodDept. Of BotanyM. P. A. S. C. College, Panvel. Date – 09/01/2016

2. Definition The culturing of pollen grains or young anthers to produce haploid plants by tissue culture is called anther culture.

3. Anther/Microspore Culture

4. Pollen Culture • Flowering plant — inflorescence — flower buds — put the flower buds in 5% teepol solution — washing in running tap water — Laminar air flow —surface sterilization — washing in S/D — culture of isolated microspore (pollen) — split the flower bud — stamen filament discard — Anther — Excised anther — Smear the anther — filter the microspore — pellet — resuspension in fresh medium — microspore —put microspore in medium — Embryogenesis — Regenerated plantlet —Regenerated haploid plant in pot.

5. Application of Anther or Pollen culture • Utility of anther and pollen culture for basic research • Use of anther and pollen culture for mutation study • Use of haploid for cryogenic study • Use of anther and pollen culture for plant breeding and crop improvement • Application of haploid culture for horticulture plants. • Anther culture and alkaloid content • Haploid culture and molecular biology. • Anther and pollen culture have prove very promising technique to obtain commercial varieties economically important crops like Rice, Wheat, Pepper, Coffee, Potato, grapes, mustard, pepper, coconut, and rubber plants. • A single anther produces a large quantity of pollen grains and in turn each of the pollen grains is capable to produce new plant thus a very large number of plant can be produced in a short period.

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