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Enzyme Kinetics and Inhibition: Mechanisms, Rates, and Plots

This lecture covers the concepts of enzyme kinetics, including the Michealis-Menten equation, Lineweaver-Burk plot, enzyme inhibition, and different types of inhibition mechanisms.

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Enzyme Kinetics and Inhibition: Mechanisms, Rates, and Plots

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  1. Lecture 15 Tuesday 3/4/08 Enzymes Michealis-Menten Kinetics Lineweaver-Burk Plot Enzyme Inhibition

  2. Last time 2/21/08

  3. Vmax=kcat* Et • Turnover Number: kcat Number of substrate molecules (moles) converted to product in a given time (s) on a single enzyme molecule (molecules/molecule/time) H2O2 + E  H2O + O + E 40,000,000 molecules of H2O2converted to product per second on a single enzyme molecule.

  4. Enzymes, Inhibition S + E → E + P 1) Mechanism and active intermediate (E·S) 2) Rates 3) Net Rates

  5. 4) Pseudo Steady-State

  6. Vmax km=S1/2 therefore km is the concentration at which the rate is half the maximum rate -rs S1/2 (Michaelis-Menten plot)

  7. Competitive Inhibition k1 k3 • E + S E·S E + P • + I E · I • Mechanisms • Rates k2 k4 k5

  8. From before (no competition): Competition No competition Intercept does not change, slope increases as inhibitor concentration increases

  9. Uncompetitive Inhibition k1 k3 E + S E·S P E · S· I k2 k4 I k5

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