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Introduction to Biotechnology!

Introduction to Biotechnology!. Learning Outcomes. Describe the science of biotechnology and identify its product domains Give examples of careers and job responsibilities associated with biotechnology

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Introduction to Biotechnology!

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  1. Introduction to Biotechnology!

  2. Learning Outcomes • Describe the science of biotechnology and identify its productdomains • Give examples of careers and job responsibilities associated with biotechnology • Outline the steps in producing and delivering a product made through recombinant DNA technology • Describe how scientific methodologies are used to conduct experiments and develop products • Apply the strategy for values clarification to bioethical issues

  3. Defining Biotechnology Biotechnology- the study and manipulation of living things or their component molecules, cells, tissues, or organs.

  4. The beginning of biotechnology • Humans have been manipulating living things for thousands of years • Selective breeding- manipulating living things with desired characteristics • Why? • Examples?

  5. Beginning of Biotechnology • Over 100 breeds of dogs created through selective breeding

  6. Beginning of Biotechnology • Different varieties of apples commonly found in grocery stores

  7. Beginning of Biotechnology • Range of size, color, and fragrance for a variety of roses

  8. Beginning of Biotechnology • Cows, goats, sheep, and chickens for milk, meat, and egg production

  9. Beginning of Biotechnology • Fermentation of foods and beverages

  10. The 1970’s • Scientists began experimenting with molecules, cells, tissues, and organs (moving away from entire organisms). WHY? • New technologies are applied to the research and development of products from plant and animal tissues • The term “Biotechnology” was coined

  11. Levels of Biological Organization • Cells of multicellular organisms are usually grouped into functional units: • Tissues • Organs • Cells are the smallest units of life. Some cells contain even smaller, nonliving units.

  12. Biotechnology today • Focuses on DNA Deoxyribonucleic Acid- a double-stranded helical molecule that stores genetic information for the production of all the organism’s proteins

  13. The Central Dogma of Biology. The Central Dogma of Biology states that DNA codes for RNA and that RNA codes for proteins (DNA -> mRNA -> proteins). Moving genes into cells to produce new proteins is the basic principle in genetic engineering.

  14. Recent uses of biotechnology • Insulin- made in bacteria cells to treat diabetes • Originally insulin was harvested from the pancreas of a slaughtered animal for treatment

  15. Recent uses of biotechnology • Proteases- proteins that break down other proteins • Commonly used in stain removal products

  16. Recent uses of biotechnology • Antibiotics- proteins developed by the immune system that recognize a specific molecule (antigen) • Used to fight diseases

  17. Recent uses of biotechnology • Indiage- protein (enzyme) that causes denim to fade to produce “stonewashed” appearance

  18. Recent uses of Biotechnology • Mouse cells “tricked” into growing outer portion of a human ear which can be surgically transferred to a human patient

  19. Recent Advances in Biotechnology come from Manipulation of DNA!What do you think of when you hear this?

  20. Techniques used to manipulate DNA • Recombinant DNA (rDNA) technology- cutting and recombining DNA molecules E. Coli transformation

  21. Techniques used to manipulate DNA • Polymerase Chain Reaction (PCR)- copying short pieces of DNA • Amplifies DNA sample

  22. Techniques used to manipulate DNA • Cloning- producing identical organisms

  23. Who is practicing Biotechnology?

  24. The Biotechnology Workplace • Biotechnology Companies - goal is to produce and sell commercial “for-profit” products • Staff- scientists, researchers, lab technicians, manufacturing, marketing, sales • Universities and Government Labs - conduct “pure science” research, nonprofit • Report results in scientific journals or meetings for the “public good”

  25. Domains of Biotechnology. The major domains of biotechnology include 1) industrial and environmental; 2) medical/pharmaceutical; 3) agricultural; and 4) diagnostic/research

  26. Government Agencies • CDC Centers for Disease Control and Prevention-national research center for developing and applying disease prevention and control, environmental health, and health promotion and education activities to improve public health • NIH National Institutes of Health- the federal agency that funds and conducts biomedical research

  27. Other institutions • DNA fingerprinting used by: • Forensic scientists within police departments to identify suspects • Ecologists to determine parentage of endangered species for breeding purposes • Wildlife biologists/customs agents to identify illegally poached or transported animals (bear gall bladders, rhinoceros horns, ivory from tusks, exotic birds) • Evolutionary biologists to determine relatedness of organisms (red panda is more closely related to the raccoon than it is to the black and white panda bear)

  28. The Increasing Variety of Biotechnology Products “In the past 100 years, scientists have increased the pace of searching for products that improve the quality of life.” As the methods of manipulating livings have become more sophisticated, the number and variety of biological products have increased at an incredible rate

  29. Techniques That Make Manipulation Possible • Restriction enzymes – cuts nucleotide sequence at specific sites on DNA molecule • DNA ligase – enzyme that pastes DNA molecules together allowing for new combinations Together these allow for us to make recombinant DNA molecules!

  30. Techniques That Make Manipulation Possible • Plasmids – tiny circular pieces of DNA usually from bacteria that is used to insert recombinant DNA into an organism

  31. rDNA to GMO Restriction enzymes, DNA ligase, and plasmids • Recombinant DNA – DNA created from two or more sources • Genetically modified organism (GMO) – organism that contains DNA from another organism and produces new proteins encoded on the acquired DNA leads to

  32. What is Genetic Engineering? • Genetic engineering refers to the modification of genetic material to achieve specific goals • We can modify organisms to express genes they never had and make proteins they never have before

  33. Examples of GMOs Gene Engineered Plant. Scientists have learned how to insert genes that code for certain traits and transfer them from one species to another. The organism that gets the new genes will then have the potential to express the new traits coded in the newly acquired genes.

  34. foods animals Examples of GMOs

  35. Examples of GMOs Producing Genetically Engineered t-PA. Humans make only a small amount of human tissue plasminogen activator (t-PA) naturally. By genetically modifying Chinese hamster ovary (CHO) cells, scientists can make large amounts of t-PA for therapeutic purposes, such as to clear blood vessels in the event of a heart attack or stroke.

  36. Examples of GMOs • Recombinant DNA and genetic engineering produces 100’s of products

  37. The Human Genome Project • Determined the human DNA sequence • Scientists now work on: • Identifying all of the genes • Determining their functions • Understanding how and when genes are turned on and off • What can this project do to advance biotechnology?

  38. Developing Ideas for New Products How Companies Select Products to Manufacture Each biotechnology company usually specializes in a group of similar products • Plant products • Fermentation equipment • Viral therapies • DNA sequencers for research • Enzymes for food processing • Ideas come from many sources: • What are some?

  39. “The Product Pipeline” Step 1: Product Development Plan Before going into research and development, company officials must determine whether or not it is worth the investment of company resources. • Product Development Plan usually includes the following criteria: • Does the product meet a critical need? Who will use the product? • Is the market large enough to produce enough sales? How many customers are there? • Do preliminary data support that the product will work? Will the product do what the company claims? • Can patent protection be secured? Can the company prevent other companies from producing it? • Can the company make a profit on the product? How much will it cost to make it? How much can it be sold for?

  40. “The Product Pipeline” Step 2: Research and Development While the product is in the product pipeline, it is constantly being evaluated by the Product Development Plan (10 to 15 years) • Research: • Is it feasible to produce new medicine in sufficient amounts? • Is it safe? • Is there proof of efficacy? (product does what it claims to do) • Is it stable over time? • Clinical Development: • Small then large scale production • Clinical trials- strict testing that evaluates effectiveness • Drugs must pass 3 phases of clinical trials with 1000’s of patients before permission from FDA to take product to market Situations That End Product Development • Product development is stopped if testing shows the product is not effective. • When this happens, companies can lose millions of dollars and years of research and development time.

  41. Federal agencies governing product development • Food and Drug Administration (FDA)-regulates the use and production of food, feed, food additives, veterinary drugs, human drugs, and medical devices • Environmental Protection Agency (EPA)-enforces environmental laws including the use and production of microorganisms, herbicides, pesticides, and genetically modified microorganisms • United States Department of Agriculture (USDA)-regulates the use and production of plants, plant products, plant tests, veterinary supplies and medications, and genetically modified plants and animals

  42. Regulations Governing Product Development New Biotech Drug Approvals. Even with all the government regulations, the number of new drugs approved for market increased nearly seven times in the 10 years between 1990 and 2000.

  43. Doing Biotechnology Students are often taught that there is a “scientific method.” What are the steps? Lets Define some key terms

  44. Fact vs. Inference FACT INFERENCE Educated guess as to what happened based on facts (observations) Conclusion deduction Judgment Goes beyond what one observes; may concern the past, present or future • An Observation • a thing that has been done • something shown to exist • something known to have existed • Confined to what one observes; cannot be made about the future

  45. What is this item?List some facts.

  46. IPOD Touch • Magnifying lens picture may have told you it could surf the web • What does the star represent? • Other Facts?

  47. What are the facts? • Deals with Algebra • Its hard • It has numbers on the cover • 1 & 2 • 1 & 3 • 2 & 3 • None of the above

  48. What are the facts? • Deals with Algebra • Its hard • It has numbers on the cover • 1 & 2 • 1 & 3 • 2 & 3 • None of the above

  49. What are the inferences? • He has something to do with baseball • He is wearing a uniform • He has gloves on • He just hit a ball • 1 & 2 • 1 & 4 • 3 & 4

  50. What are the inferences? • He has something to do with baseball • He is wearing a uniform • He has gloves on • He just hit a ball • 1 & 2 • 1 & 4 • 3 & 4

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