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Unit 1—Ch 2. Biology as a Science. Microscopes were 1 st introduced in the 1600’s by Galileo. Shown here is Robert Hooke’s simple microscope. Microscopy timeline. http://micro.magnet.fsu.edu/primer/anatomy/introduction.html.
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Unit 1—Ch 2 Biology as a Science
Microscopes were 1st introduced in the 1600’s by Galileo. Shown here is Robert Hooke’s simple microscope. Microscopy timeline http://micro.magnet.fsu.edu/primer/anatomy/introduction.html http://www.moonmentum.com/blog/wp-content/uploads/2010/03/Robert-Hooke.jpg
Microscopes • use to magnify & to increase resolving power • common lab microscopes uses light passing through an object & lens to produce a larger image • Simple light microscope • Compound light microscope
Compound Light Microscopes • Uses light & 2 or more glass lenses to focus light • Total magnification • Eyepiece • Objectives • Total magnification up to 2000x • Lower resolving power • live & dead specimens • COLOR!!! • Many are portable
stoma Light Microscopes • Shown here are an amoeba (left), stoma in leaf (center) & onion cells (right). Stains help us to see some specimens better…
Microscope parts quiz Parts of a compound light microscope Interactive microscope guide
Virtual Microscope Microscope parts http://www.science.smith.edu/departments/Biology/Bio111/Labmanual/scope.jpg
Electron Microscopes • developed in 1960’s • beam of electrons & magnetic lenses produce enlarged image • only dead specimens • greater resolving power & magnification than light microscope • 10,000-200,000x
Transmission Electron Microscope (TEM) • Electrons pass through very thin non-living specimen • Black & white, 2-D, image of inside • Specimen frozen or embedded in plastic first • resolution: 0.5 - 10 nm • magnification: > 1,000,000x TEM simulator Preparation of a specimen http://nobelprize.org/educational/physics/microscopes/tem/index.html
Scanning Electron Microscope (SEM) • electrons bounce off surface of non-living specimen • Black & white, 3-D, image of surface • Micrographs can be color enhanced using computer • Magnification < TEM • 100,000x plus • Resolving power < TEM SEM Interactive Virtual SEM
The samples shown above are (clockwise from top left) an ant (Lasius flavus), passion fruit (Passiflora caerulea) pollen, a freshwater shrimp parasite (Epistillis sp.), sunflower (Helianthus anuus) pollen and central, agreenbottle (Lucilia sericata) foot.
Comparison of Microscopes http://universe-review.ca/R11-13-microscopes.htm
Science & Technology • Science- is an organized process that produces a body of knowledge about nature. • Based on observation of phenomenon or their effects & experimentation in order to attempt to explain the cause of those effects • Goal is to produce useful models of reality • Technology-Applying knowledge to real problems
The Scientific Method http://www.sciencebuddies.org/science-fair-projects/overview_scientific_method2.gif
The Scientific Method • Observations • question or problem to investigate • background information • hypothesis is formed • “If _(IV)_, then _(DV)_, because __.” • Test hypothesis controlled experiment
Controlled experiment • to be valid has to have these two parts!!!!! • 1. Tests one variable, all others kept same • Independent Variable (IV) • Dependent Variable (DV) • Controlled Variables or Constants (Cv) • 2. Two groups must be tested. • control group (set-up) • experimental group (set-up) • Multiple Trials (replicates) • Ensure results are accurate
The Scientific Method • Make observations & collect data • data tables • Interpret data & analyze results • graphs • Draw conclusions • Support/reject hypothesis (NOT prove/disprove) • Conduct additional experiments (revise or discard hypothesis if rejected) • Communicate results • Report & publish
Which brand of mouthwash gets rid of stinky breath the best? • Independent Variable- • Dependent Variable- • Controlled Variables- • Experimental Group(s)- • Control Group-
Theories & Laws • Can you prove anything in science? • Theory • hypothesis becomes theory when it has been supported repeatedly by experimental evidence • ex. evolution, natural selection, relativity • used to explain laws • as close to complete explanation as science can offer • can change/be refuted based on new evidence.
Examples of Theories • Darwin’s finches ex. of his theory of evolution through natural selection • Lack of proper experimentation led to belief in abiogenesis (spontaneous generation) • Controlled experiments like F. Redi’s “maggots from meat” experiment refuted this theory.
Theories & Laws • Laws • Statements about events that always occur in nature • Does not give mechanism or explanation of phenomenon • often expressed as a numerical equation
Examples of Laws • Newton • Nicolas Steno’s Law of Superposition • Fossils in lowest layer are oldest, in uppermost layer are youngest